302354

antibody from BioLegend

Targeting: MS4A1 B1, Bp35, CD20, MS4A2

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

302354

Provider

BioLegend

Proper citation

BioLegend Cat#302354, RRID:AB_2565619

Product name

Alexa Fluor® 594 anti-human CD20

Antibody type

Monoclonal

Antigen

Human tonsillar B cells

Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca²⁺ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

2H7

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA) then blocked with 5% FBS. The cells were then stained with 10 µg/ml CD20 (clone 2H7) Alexa Fluor® 594 (red), CD3 (clone UCHT1) Alexa Fluor® 647 (yellow), and CD14 (clone HCD14) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Conjugate

Yellow dye