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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- UM800009 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800009, RRID:AB_2629126
- Product name
- Purified CD20 (MS4A1) mouse monoclonal antibody,clone UMAB58
- Antibody type
- Monoclonal
- Description
- Purified CD20 (MS4A1) mouse monoclonal antibody,clone UMAB58
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MS4A1
- Isotype
- IgG
- Antibody clone number
- UMAB58
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Jurkat cells using anti-CD20 mouse monoclonal antibody (UM800009, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD20 clone UMAB58 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800009 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber . The image shows the germinal and non germinal cells have strong cytoplasmic and memeranous staining.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 clone UMAB58 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800009 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber . The image shows the cells in the white pulp have strong cytoplasmic and membraneous staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MS4A1 mouse monoclonal antibody (UM800009). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
