343522

antibody from BioLegend

Targeting: CD34

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

343522

Provider

BioLegend

Proper citation

BioLegend Cat#343522, RRID:AB_2228973

Product name

PerCP/Cy5.5 anti-human CD34

Antibody type

Monoclonal

Antigen

CD34

Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Far-Red dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

A promoter DNA demethylation landscape of human hematopoietic differentiation.

Calvanese V, Fernández AF, Urdinguio RG, Suárez-Alvarez B, Mangas C, Pérez-García V, Bueno C, Montes R, Ramos-Mejía V, Martínez-Camblor P, Ferrero C, Assenov Y, Bock C, Menendez P, Carrera AC, Lopez-Larrea C, Fraga MF
Nucleic acids research 2012 Jan;40(1):116-31

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood leukocytes stained with CD45 FITC and 581 PerCP/Cy5.5 (top) or mouse IgG1,κ PerCP/Cy5.5 isotype control (bottom). Cytogram gated for total live CD14 negative lymphocyte population