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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 318012 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#318012, RRID:AB_2650948
- Product name
- Alexa Fluor® 647 anti-human CD99
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antibody clone number
- HCD99
- Vial size
- 100 µg
- Concentration
- 0.50 mg/mL
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- Main image
- Experimental details
- Human paraffin-embedded pancreas tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-human CD99 (clone HCD99) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
- Conjugate
- Red dye
