GTX113219

antibody from GeneTex

Targeting: KRT5 EBS2, KRT5A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113219

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113219, RRID:AB_10623180

Product name

Cytokeratin 5 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

MAPK Pathway Involved in Epidermal Terminal Differentiation of Normal Human Epidermal Keratinocytes.

Meng X, Qiu L, Song H, Dang N
Open medicine (Warsaw, Poland) 2018;13:189-195

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Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.

Wang S, Qiu L, Meng X, Dang N
Medecine sciences : M/S 2018 Oct;34 Focus issue F1:94-98

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LncRNA expression profile of ΔNp63α in cervical squamous cancers and its suppressive effects on LIF expression.

Qian L, Xu F, Wang X, Jiang M, Wang J, Song W, Wu D, Shen Z, Feng D, Ling B, Cheng Y, Xiao W, Shan G, Zhou Y
Cytokine 2017 Aug;96:114-122

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MAOA-a novel decision maker of apoptosis and autophagy in hormone refractory neuroendocrine prostate cancer cells.

Lin YC, Chang YT, Campbell M, Lin TP, Pan CC, Lee HC, Shih JC, Chang PC
Scientific reports 2017 Apr 12;7:46338

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Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.

Dang NN, Pang SG, Song HY, An LG, Ma XL
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2015 Jan;48(1):39-45

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Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.

Dang N, Pang S, Song H, Bian H, Zhang X, An L, Ma X
Molecular biology reports 2015 Feb;42(2):337-43

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: HCT116 7.5% SDS PAGE Cytokeratin 5 antibody GTX113219 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 5 antibody (GTX113219) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 5(GTX113219) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cytosol and membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Cytokeratin 5 antibody (GTX113219) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cytokeratin 5 antibody detects Cytokeratin 5 protein at cell membrane and cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cytokeratin 5 antibody (GTX113219) diluted at 1:500.