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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- V3059 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Cytokeratin 5 + 8 Antibody
- Antibody type
- Monoclonal
- Antigen
- A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5 + 8 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Sheep, Xenopus
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- C-50
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Cytokeratin 5 + 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Expression of cytokeratin subtypes in intraepidermal malignancies: a guide for differentiation.
Isolation of exfoliated colonocytes from human stool as a new technique for colonic cytology.
Aslan F, Demirkesen C, Cağatay P, Tüzüner N
Journal of cutaneous pathology 2006 Aug;33(8):531-8
Journal of cutaneous pathology 2006 Aug;33(8):531-8
Isolation of exfoliated colonocytes from human stool as a new technique for colonic cytology.
Bandaletova T, Bailey N, Bingham SA, Loktionov A
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2002 Mar;110(3):239-46
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2002 Mar;110(3):239-46
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).
