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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- NBP1-97730 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 5/8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. RCK102 is reacting with cytokeratin 5 and cytokeratin 8. This monoclonal antibody recognizes virtually all epithelial tissues and carcinomas.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Hamster, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Long-segmental tracheal reconstruction in rabbits with pedicled Tissue-engineered trachea based on a 3D-printed scaffold.
Rapid nodal staging of head and neck cancer surgical specimens with flow cytometric analysis.
Gao B, Jing H, Gao M, Wang S, Fu W, Zhang X, He X, Zheng J
Acta biomaterialia 2019 Oct 1;97:177-186
Acta biomaterialia 2019 Oct 1;97:177-186
Rapid nodal staging of head and neck cancer surgical specimens with flow cytometric analysis.
Häyry V, Kågedal Å, Hjalmarsson E, Neves da Silva PF, Drakskog C, Margolin G, Georén SK, Munck-Wikland E, Winqvist O, Cardell LO
British journal of cancer 2018 Feb 6;118(3):421-427
British journal of cancer 2018 Feb 6;118(3):421-427
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human liver hepatocytes and bile ducts
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Frozen sections of human urinary bladder epithelium
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on NTERA-2 cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 5/8 Antibody (RCK102) [NBP1-97730] - An intracellular stain was performed on HeLa cells with Cytokeratin 5/8 (RCK102) antibody NBP1-97730AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
