LS-C407795

antibody from LSBio

Targeting: EIF6 b(2)gcn, EIF3A, ITGB4BP, p27BBP

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C407795

Provider

LSBio

Product name

EIF6 Antibody (aa66-210) LS-C407795

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.