Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G6PD Recombinant Rabbit Monoclonal Antibody (JA31-40)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JA31-40
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G6PD in A549 cell lysate using a G6PD Monoclonal antibody (Product # MA5-32681) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of G6PD was achieved by transfecting HeLa cells with G6PD specific siRNAs (Silencer® select Product # s5447). Western blot analysis (Fig. a) was performed whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with G6PD Recombinant Rabbit Monoclonal Antibody (Product # MA5-32681, 1:1000) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to G6PD.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-G6PD Recombinant Rabbit Monoclonal Antibody (Product # MA5-32681) and a 59 kDa band corresponding to G6PD was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), SW480 (Lane 3), MCF7 (Lane 4), HeLa (Lane 5), 3T3-L1 (Lane 6), 3T3-L1 differentiated to Adipocytes (Lane 7) and K-562 (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). This product does not show reactivity to mouse G6PD, as no band was observed in mouse 3T3-L1 cell line when differentiated to adipocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of G6PD in MCF-7 cells using a G6PD Monoclonal antibody (Product # MA5-32681) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of G6PD of paraffin-embedded Mouse testes tissue using a G6PD Monoclonal antibody (Product #MA5-32681). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of G6PD of paraffin-embedded Human liver tissue using a G6PD Monoclonal antibody (Product #MA5-32681). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of G6PD of paraffin-embedded Human stomach cancer tissue using a G6PD Monoclonal antibody (Product #MA5-32681). Counter stained with hematoxylin..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of G6PD of paraffin-embedded Mouse skeletal muscle tissue using a G6PD Monoclonal antibody (Product #MA5-32681). Counter stained with hematoxylin..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of G6PD in Hela cells using a G6PD Monoclonal Antibody (Product # MA5-32681) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).