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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-789 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-789, RRID:AB_631274
- Product name
- Anti-MYC
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0.
The herpesvirus associated ubiquitin specific protease, USP7, is a negative regulator of PML proteins and PML nuclear bodies.
Intergenic transcription causes repression by directing nucleosome assembly.
USP7/HAUSP promotes the sequence-specific DNA binding activity of p53.
Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages.
Setdb1 histone methyltransferase regulates mood-related behaviors and expression of the NMDA receptor subunit NR2B.
Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing.
Hepatitis B virus X protein impairs hepatic insulin signaling through degradation of IRS1 and induction of SOCS3.
Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus.
Estrogen receptor-alpha as a drug target candidate for preventing lung inflammation.
Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase.
PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.
Drosophila stathmins bind tubulin heterodimers with high and variable stoichiometries.
The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradation.
SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast.
Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.
Developmentally regulated MAPK pathways modulate heterochromatin in Saccharomyces cerevisiae.
Efficient chromosome biorientation and the tension checkpoint in Saccharomyces cerevisiae both require Bir1.
CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.
Rac1-induced cell migration requires membrane recruitment of the nuclear oncogene SET
Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+.
Kamakura M, Goshima F, Luo C, Kimura H, Nishiyama Y
Virology journal 2011 May 25;8:257
Virology journal 2011 May 25;8:257
The herpesvirus associated ubiquitin specific protease, USP7, is a negative regulator of PML proteins and PML nuclear bodies.
Sarkari F, Wang X, Nguyen T, Frappier L
PloS one 2011 Jan 31;6(1):e16598
PloS one 2011 Jan 31;6(1):e16598
Intergenic transcription causes repression by directing nucleosome assembly.
Hainer SJ, Pruneski JA, Mitchell RD, Monteverde RM, Martens JA
Genes & development 2011 Jan 1;25(1):29-40
Genes & development 2011 Jan 1;25(1):29-40
USP7/HAUSP promotes the sequence-specific DNA binding activity of p53.
Sarkari F, Sheng Y, Frappier L
PloS one 2010 Sep 27;5(9):e13040
PloS one 2010 Sep 27;5(9):e13040
Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages.
Mackellar DC, O'Neill MT, Aly AS, Sacci JB Jr, Cowman AF, Kappe SH
Eukaryotic cell 2010 May;9(5):784-94
Eukaryotic cell 2010 May;9(5):784-94
Setdb1 histone methyltransferase regulates mood-related behaviors and expression of the NMDA receptor subunit NR2B.
Jiang Y, Jakovcevski M, Bharadwaj R, Connor C, Schroeder FA, Lin CL, Straubhaar J, Martin G, Akbarian S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 May 26;30(21):7152-67
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 May 26;30(21):7152-67
Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing.
Mimura S, Yamaguchi T, Ishii S, Noro E, Katsura T, Obuse C, Kamura T
The Journal of biological chemistry 2010 Mar 26;285(13):9858-67
The Journal of biological chemistry 2010 Mar 26;285(13):9858-67
Hepatitis B virus X protein impairs hepatic insulin signaling through degradation of IRS1 and induction of SOCS3.
Kim K, Kim KH, Cheong J
PloS one 2010 Mar 23;5(3):e8649
PloS one 2010 Mar 23;5(3):e8649
Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus.
Jang IC, Henriques R, Seo HS, Nagatani A, Chua NH
The Plant cell 2010 Jul;22(7):2370-83
The Plant cell 2010 Jul;22(7):2370-83
Estrogen receptor-alpha as a drug target candidate for preventing lung inflammation.
Vegeto E, Cuzzocrea S, Crisafulli C, Mazzon E, Sala A, Krust A, Maggi A
Endocrinology 2010 Jan;151(1):174-84
Endocrinology 2010 Jan;151(1):174-84
Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase.
Niida H, Katsuno Y, Sengoku M, Shimada M, Yukawa M, Ikura M, Ikura T, Kohno K, Shima H, Suzuki H, Tashiro S, Nakanishi M
Genes & development 2010 Feb 15;24(4):333-8
Genes & development 2010 Feb 15;24(4):333-8
PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells.
Halaban R, Zhang W, Bacchiocchi A, Cheng E, Parisi F, Ariyan S, Krauthammer M, McCusker JP, Kluger Y, Sznol M
Pigment cell & melanoma research 2010 Apr;23(2):190-200
Pigment cell & melanoma research 2010 Apr;23(2):190-200
Drosophila stathmins bind tubulin heterodimers with high and variable stoichiometries.
Lachkar S, Lebois M, Steinmetz MO, Guichet A, Lal N, Curmi PA, Sobel A, Ozon S
The Journal of biological chemistry 2010 Apr 9;285(15):11667-80
The Journal of biological chemistry 2010 Apr 9;285(15):11667-80
The group migration of Dictyostelium cells is regulated by extracellular chemoattractant degradation.
Garcia GL, Rericha EC, Heger CD, Goldsmith PK, Parent CA
Molecular biology of the cell 2009 Jul;20(14):3295-304
Molecular biology of the cell 2009 Jul;20(14):3295-304
SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast.
Mimura S, Komata M, Kishi T, Shirahige K, Kamura T
The EMBO journal 2009 Dec 2;28(23):3693-705
The EMBO journal 2009 Dec 2;28(23):3693-705
Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.
Archer SK, Luu VD, de Queiroz RA, Brems S, Clayton C
PLoS pathogens 2009 Aug;5(8):e1000565
PLoS pathogens 2009 Aug;5(8):e1000565
Developmentally regulated MAPK pathways modulate heterochromatin in Saccharomyces cerevisiae.
Mazor Y, Kupiec M
Nucleic acids research 2009 Aug;37(14):4839-49
Nucleic acids research 2009 Aug;37(14):4839-49
Efficient chromosome biorientation and the tension checkpoint in Saccharomyces cerevisiae both require Bir1.
Makrantoni V, Stark MJ
Molecular and cellular biology 2009 Aug;29(16):4552-62
Molecular and cellular biology 2009 Aug;29(16):4552-62
CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.
Nishitani H, Shiomi Y, Iida H, Michishita M, Takami T, Tsurimoto T
The Journal of biological chemistry 2008 Oct 24;283(43):29045-52
The Journal of biological chemistry 2008 Oct 24;283(43):29045-52
Rac1-induced cell migration requires membrane recruitment of the nuclear oncogene SET
Jean Paul ten Klooster, Ingrid v Leeuwen, Nina Scheres, Eloise C Anthony, Peter L Hordijk
The EMBO Journal 2007 Jan;26(2):336-345
The EMBO Journal 2007 Jan;26(2):336-345
Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+.
Freeman-Cook LL, Gómez EB, Spedale EJ, Marlett J, Forsburg SL, Pillus L, Laurenson P
Genetics 2005 Mar;169(3):1243-60
Genetics 2005 Mar;169(3):1243-60
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lateral ventricle shows distinct nucleolar positivity in neuronal cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
