AHO0062
antibody from Invitrogen Antibodies
Targeting: MYC
bHLHe39, c-Myc, MYCC
Antibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [4]
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Validation data
Reference
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- Product number
- AHO0062 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects an epitope at aa 410-419. Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Recommended positive controls include Raji cells and human breast carcinoma.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9E10.3
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Lineage-specific selection and the evolution of virulence in the Candida clade.
Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport.
Identification of anti-tumoral feedback loop between VHLα and hnRNPA2B1 in renal cancer.
Dual regulation of Arabidopsis AGO2 by arginine methylation.
JQ1 Induces DNA Damage and Apoptosis, and Inhibits Tumor Growth in a Patient-Derived Xenograft Model of Cholangiocarcinoma.
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis.
CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation.
Ssn6 Defines a New Level of Regulation of White-Opaque Switching in Candida albicans and Is Required For the Stochasticity of the Switch.
Identification and Characterization of Wor4, a New Transcriptional Regulator of White-Opaque Switching.
The BET bromodomain inhibitor JQ1 suppresses growth of pancreatic ductal adenocarcinoma in patient-derived xenograft models.
Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.
Nuclear retention of full-length HTT RNA is mediated by splicing factors MBNL1 and U2AF65.
Coordinated regulation of photosynthesis in rice increases yield and tolerance to environmental stress.
Tubulin polymerization promoting protein 1 (TPPP1) increases β-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells.
Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.
Biofilm matrix regulation by Candida albicans Zap1.
NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes.
Prognostic significance of c-Met expression in glioblastomas.
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Human melanocortin receptor 2 expression and functionality: effects of protein kinase A and protein kinase C on desensitization and internalization.
c-Myc-regulated microRNAs modulate E2F1 expression.
A core function for p120-catenin in cadherin turnover.
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity.
The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells.
PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells.
Early and persistent bone marrow hematopoiesis defect in simian/human immunodeficiency virus-infected macaques despite efficient reduction of viremia by highly active antiretroviral therapy during primary infection.
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Singh-Babak SD, Babak T, Fraser HB, Johnson AD
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar 23;118(12)
Proceedings of the National Academy of Sciences of the United States of America 2021 Mar 23;118(12)
Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport.
Jiang T, Mu B, Zhao R
Journal of experimental botany 2020 Dec 31;71(22):7073-7087
Journal of experimental botany 2020 Dec 31;71(22):7073-7087
Identification of anti-tumoral feedback loop between VHLα and hnRNPA2B1 in renal cancer.
Liu Y, Zhang H, Li X, Zhang C, Huang H
Cell death & disease 2020 Aug 11;11(8):688
Cell death & disease 2020 Aug 11;11(8):688
Dual regulation of Arabidopsis AGO2 by arginine methylation.
Hu P, Zhao H, Zhu P, Xiao Y, Miao W, Wang Y, Jin H
Nature communications 2019 Feb 19;10(1):844
Nature communications 2019 Feb 19;10(1):844
JQ1 Induces DNA Damage and Apoptosis, and Inhibits Tumor Growth in a Patient-Derived Xenograft Model of Cholangiocarcinoma.
Garcia PL, Miller AL, Gamblin TL, Council LN, Christein JD, Arnoletti JP, Heslin MJ, Reddy S, Richardson JH, Cui X, van Waardenburg RCAM, Bradner JE, Yang ES, Yoon KJ
Molecular cancer therapeutics 2018 Jan;17(1):107-118
Molecular cancer therapeutics 2018 Jan;17(1):107-118
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis.
Macia MS, Halbritter J, Delous M, Bredrup C, Gutter A, Filhol E, Mellgren AEC, Leh S, Bizet A, Braun DA, Gee HY, Silbermann F, Henry C, Krug P, Bole-Feysot C, Nitschké P, Joly D, Nicoud P, Paget A, Haugland H, Brackmann D, Ahmet N, Sandford R, Cengiz N, Knappskog PM, Boman H, Linghu B, Yang F, Oakeley EJ, Saint Mézard P, Sailer AW, Johansson S, Rødahl E, Saunier S, Hildebrandt F, Benmerah A
American journal of human genetics 2017 Feb 2;100(2):323-333
American journal of human genetics 2017 Feb 2;100(2):323-333
CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation.
Ramena G, Yin Y, Yu Y, Walia V, Elble RC
PloS one 2016;11(3):e0147489
PloS one 2016;11(3):e0147489
Ssn6 Defines a New Level of Regulation of White-Opaque Switching in Candida albicans and Is Required For the Stochasticity of the Switch.
Hernday AD, Lohse MB, Nobile CJ, Noiman L, Laksana CN, Johnson AD
mBio 2016 Jan 26;7(1):e01565-15
mBio 2016 Jan 26;7(1):e01565-15
Identification and Characterization of Wor4, a New Transcriptional Regulator of White-Opaque Switching.
Lohse MB, Johnson AD
G3 (Bethesda, Md.) 2016 Jan 15;6(3):721-9
G3 (Bethesda, Md.) 2016 Jan 15;6(3):721-9
The BET bromodomain inhibitor JQ1 suppresses growth of pancreatic ductal adenocarcinoma in patient-derived xenograft models.
Garcia PL, Miller AL, Kreitzburg KM, Council LN, Gamblin TL, Christein JD, Heslin MJ, Arnoletti JP, Richardson JH, Chen D, Hanna CA, Cramer SL, Yang ES, Qi J, Bradner JE, Yoon KJ
Oncogene 2016 Feb 18;35(7):833-45
Oncogene 2016 Feb 18;35(7):833-45
Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.
DeRussy BM, Boland MT, Tandon R
Journal of virology 2016 Aug 15;90(16):7109-7117
Journal of virology 2016 Aug 15;90(16):7109-7117
Nuclear retention of full-length HTT RNA is mediated by splicing factors MBNL1 and U2AF65.
Sun X, Li PP, Zhu S, Cohen R, Marque LO, Ross CA, Pulst SM, Chan HY, Margolis RL, Rudnicki DD
Scientific reports 2015 Jul 28;5:12521
Scientific reports 2015 Jul 28;5:12521
Coordinated regulation of photosynthesis in rice increases yield and tolerance to environmental stress.
Ambavaram MM, Basu S, Krishnan A, Ramegowda V, Batlang U, Rahman L, Baisakh N, Pereira A
Nature communications 2014 Oct 31;5:5302
Nature communications 2014 Oct 31;5:5302
Tubulin polymerization promoting protein 1 (TPPP1) increases β-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells.
Schofield AV, Gamell C, Bernard O
Biochemical and biophysical research communications 2013 Jul 12;436(4):571-7
Biochemical and biophysical research communications 2013 Jul 12;436(4):571-7
Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
Novellino L, Rossi RL, Bonino F, Cavallone D, Abrignani S, Pagani M, Brunetto MR
PloS one 2012;7(3):e31952
PloS one 2012;7(3):e31952
Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.
Giaginis C, Politi E, Alexandrou P, Sfiniadakis J, Kouraklis G, Theocharis S
Pathology oncology research : POR 2012 Oct;18(4):875-83
Pathology oncology research : POR 2012 Oct;18(4):875-83
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Huang YS, Chiang NY, Hu CH, Hsiao CC, Cheng KF, Tsai WP, Yona S, Stacey M, Gordon S, Chang GW, Lin HH
Molecular and cellular biology 2012 Apr;32(8):1408-20
Molecular and cellular biology 2012 Apr;32(8):1408-20
A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.
Lanham KA, Prasch AL, Weina KM, Peterson RE, Heideman W
PloS one 2011;6(12):e28020
PloS one 2011;6(12):e28020
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Challagundla KB, Sun XX, Zhang X, DeVine T, Zhang Q, Sears RC, Dai MS
Molecular and cellular biology 2011 Oct;31(19):4007-21
Molecular and cellular biology 2011 Oct;31(19):4007-21
Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.
Yang L, Shen L, Shao Y, Zhao Q, Zhang W
Cellular immunology 2009;258(1):78-82
Cellular immunology 2009;258(1):78-82
Biofilm matrix regulation by Candida albicans Zap1.
Nobile CJ, Nett JE, Hernday AD, Homann OR, Deneault JS, Nantel A, Andes DR, Johnson AD, Mitchell AP
PLoS biology 2009 Jun;7(6):e1000133
PLoS biology 2009 Jun;7(6):e1000133
NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes.
Katzenellenbogen RA, Vliet-Gregg P, Xu M, Galloway DA
Journal of virology 2009 Jul;83(13):6446-56
Journal of virology 2009 Jul;83(13):6446-56
Prognostic significance of c-Met expression in glioblastomas.
Kong DS, Song SY, Kim DH, Joo KM, Yoo JS, Koh JS, Dong SM, Suh YL, Lee JI, Park K, Kim JH, Nam DH
Cancer 2009 Jan 1;115(1):140-8
Cancer 2009 Jan 1;115(1):140-8
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Itahana K, Zhang Y
Cancer cell 2008 Jun;13(6):542-53
Cancer cell 2008 Jun;13(6):542-53
Human melanocortin receptor 2 expression and functionality: effects of protein kinase A and protein kinase C on desensitization and internalization.
Kilianova Z, Basora N, Kilian P, Payet MD, Gallo-Payet N
Endocrinology 2006 May;147(5):2325-37
Endocrinology 2006 May;147(5):2325-37
c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT
Nature 2005 Jun 9;435(7043):839-43
Nature 2005 Jun 9;435(7043):839-43
A core function for p120-catenin in cadherin turnover.
Davis MA, Ireton RC, Reynolds AB
The Journal of cell biology 2003 Nov 10;163(3):525-34
The Journal of cell biology 2003 Nov 10;163(3):525-34
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
Kefas BA, Cai Y, Ling Z, Heimberg H, Hue L, Pipeleers D, Van de Casteele M
Journal of molecular endocrinology 2003 Apr;30(2):151-61
Journal of molecular endocrinology 2003 Apr;30(2):151-61
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
Kefas BA, Cai Y, Ling Z, Heimberg H, Hue L, Pipeleers D, Van de Casteele M
Journal of molecular endocrinology 2003 Apr;30(2):151-61
Journal of molecular endocrinology 2003 Apr;30(2):151-61
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Hitzl M, Striessnig J, Neuhuber B, Flucher BE
FEBS letters 2002 Jul 31;524(1-3):188-92
FEBS letters 2002 Jul 31;524(1-3):188-92
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Hitzl M, Striessnig J, Neuhuber B, Flucher BE
FEBS letters 2002 Jul 31;524(1-3):188-92
FEBS letters 2002 Jul 31;524(1-3):188-92
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Piechocki MP, Pilon SA, Wei WZ
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Piechocki MP, Pilon SA, Wei WZ
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Baker-LePain JC, Sarzotti M, Fields TA, Li CY, Nicchitta CV
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Baker-LePain JC, Sarzotti M, Fields TA, Li CY, Nicchitta CV
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity.
Chavand O, Spilsbury K, Rakoczy PE
Biochemistry and cell biology = Biochimie et biologie cellulaire 2001;79(1):107-12
Biochemistry and cell biology = Biochimie et biologie cellulaire 2001;79(1):107-12
The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells.
Fukumoto T, Watanabe-Fukunaga R, Fujisawa K, Nagata S, Fukunaga R
The Journal of biological chemistry 2001 Oct 19;276(42):38441-8
The Journal of biological chemistry 2001 Oct 19;276(42):38441-8
PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells.
Castoria G, Migliaccio A, Bilancio A, Di Domenico M, de Falco A, Lombardi M, Fiorentino R, Varricchio L, Barone MV, Auricchio F
The EMBO journal 2001 Nov 1;20(21):6050-9
The EMBO journal 2001 Nov 1;20(21):6050-9
Early and persistent bone marrow hematopoiesis defect in simian/human immunodeficiency virus-infected macaques despite efficient reduction of viremia by highly active antiretroviral therapy during primary infection.
Thiebot H, Louache F, Vaslin B, de Revel T, Neildez O, Larghero J, Vainchenker W, Dormont D, Le Grand R
Journal of virology 2001 Dec;75(23):11594-602
Journal of virology 2001 Dec;75(23):11594-602
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Bourgarel-Rey V, El Khyari S, Rimet O, Bordas B, Guigal N, Braguer D, Seree E, Barra Y, Briand C
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Bourgarel-Rey V, El Khyari S, Rimet O, Bordas B, Guigal N, Braguer D, Seree E, Barra Y, Briand C
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
de Caestecker MP, Yahata T, Wang D, Parks WT, Huang S, Hill CS, Shioda T, Roberts AB, Lechleider RJ
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
de Caestecker MP, Yahata T, Wang D, Parks WT, Huang S, Hill CS, Shioda T, Roberts AB, Lechleider RJ
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Cooper JK, Schilling G, Peters MF, Herring WJ, Sharp AH, Kaminsky Z, Masone J, Khan FA, Delanoy M, Borchelt DR, Dawson VL, Dawson TM, Ross CA
Human molecular genetics 1998 May;7(5):783-90
Human molecular genetics 1998 May;7(5):783-90
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Cooper JK, Schilling G, Peters MF, Herring WJ, Sharp AH, Kaminsky Z, Masone J, Khan FA, Delanoy M, Borchelt DR, Dawson VL, Dawson TM, Ross CA
Human molecular genetics 1998 May;7(5):783-90
Human molecular genetics 1998 May;7(5):783-90
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of c-Myc in Raji cells using a c-Myc monoclonal antibody (Product # AHO0062).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of c-Myc in formalin-fixed, paraffin-embedded human breast carcinoma using a c-Myc monoclonal antibody (Product # AHO0062). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 ChIP mapping of genomic Zap1 binding sites. Zap1 myc-tagged strain CJN1688 versus untagged wild-type strain DAY185 immunoprecipitation binding data were performed under biofilm conditions. The x -axis represents ORF chromosomal locations (See Dataset S6 , sheet 1 for exact location values). The y -axis is the Agilent normalized enrichment value (log2) for binding of Zap1 (See Dataset S6 , sheet 1 for exact enrichment values). Zap1-myc strain (blue line) and untagged wild-type (red line) ChIP-chip array binding data were mapped and plotted onto the chromosomes containing ZRT1 and PRA1 located on Chromosome 4 (A), ZRT2 located on Chromosome 2 (B), ZRT3 located on Chromosome 2 (C), CSH1 located on Chromosome 1 (D), IFD6 located on Chromosome 1 (E), and itself ZAP1 located on Chromosome 4 (F) using ChipView v0.954. The promoters of these genes show significant peak enrichment (determined using Agilent Chip Analytics software v1.2) for the binding of Zap1. The blue track under the peak indicates that the Agilent segment p -value (-log10) for the binding of Zap1 is significant (See Dataset S6 , sheet 1 for actual segment p -values). Genes plotted above the bold line read in the sense direction; genes plotted below the bold line read in the antisense direction. Identical binding sites with similar peak enrichment values were observed for the independently isolated Zap1 myc-tagged strain CJN1694 versus untagged wild-type strain DAY185 (unpublished data).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 ChIP mapping of genomic Zap1 binding sites. Zap1 myc-tagged strain CJN1688 versus untagged wild-type strain DAY185 immunoprecipitation binding data were performed under biofilm conditions. The x -axis represents ORF chromosomal locations (See Dataset S6 , sheet 1 for exact location values). The y -axis is the Agilent normalized enrichment value (log2) for binding of Zap1 (See Dataset S6 , sheet 1 for exact enrichment values). Zap1-myc strain (blue line) and untagged wild-type (red line) ChIP-chip array binding data were mapped and plotted onto the chromosomes containing ZRT1 and PRA1 located on Chromosome 4 (A), ZRT2 located on Chromosome 2 (B), ZRT3 located on Chromosome 2 (C), CSH1 located on Chromosome 1 (D), IFD6 located on Chromosome 1 (E), and itself ZAP1 located on Chromosome 4 (F) using ChipView v0.954. The promoters of these genes show significant peak enrichment (determined using Agilent Chip Analytics software v1.2) for the binding of Zap1. The blue track under the peak indicates that the Agilent segment p -value (-log10) for the binding of Zap1 is significant (See Dataset S6 , sheet 1 for actual segment p -values). Genes plotted above the bold line read in the sense direction; genes plotted below the bold line read in the antisense direction. Identical binding sites with similar peak enrichment values were observed for the independently isolated Zap1 myc-tagged strain CJN1694 versus untagged wild-type strain DAY185 (unpublished data).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6. Split YFP-based bimolecular fluorescence complementation and co-IP analyses. (A and B) Laser scanning microscopy images were taken from mesophyll cells of N. benthamiana transiently co-expressing YFP N- or C-half fusion proteins that are labeled as -YFP N and -YFP C respectively. Fluorescence images for YFP, chlorophyll, and the merged signals are shown. Scale bars represent 5 um. (C) Co-IP of the Arabidopsis SecY1 complex from tobacco leaves after co-infiltration and transient expression of YFP N -tagged SecY1 and GFP fusion proteins that were led by PsbO1 1-85 (lane 2) or full-length PsbO1 (PsbO1 1-332 ) (lane 3). Lane 1 is the wild-type control. YFP N was also Myc tagged. Anti-HSP90C antibody recognizes both Arabidopsis and tobacco chloroplast HSP90C. It should be noted that all full-length GFP fusion constructs are based on the pEGAD vector and there is a 27 amino acid linker region after the GFP C-terminus yielding a slightly larger GFP fusion protein than normally expected. Construct details are also shown in Fig. 1A . (D) Co-IP of the SecY1 complex from 12-day-old SECY1-T7/ secy1 Arabidopsis seedlings using anti-T7 antibody agarose. Wild-type (WT) seedlings were used as a negative control. Anti-HSP90C and anti-T7 antibodies were used to detect HSP90C and SecY1 proteins in both total input and the pull-down (IP) samples.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3. The role of Tye7 in lineage-specific selection on glycolysis. ( A ) Cells were serially diluted fivefold onto solid minimal media containing 2% glucose as a carbon source with and without antimycin A to inhibit respiration and force cells to rely on glycolysis. Plates were grown at 30 degC for 72 h and pictures were taken with identical aperture and exposure settings. The results show that Tye7 is needed in C. dubliniensis for growth on antimycin A. ( B ) Schematic of immunoprecipitation experiments performed in the interspecies hybrid where one allele of the C. dubliniensis Tye7 was epitope-tagged. The ChIP-seq reads from the tagged hybrid were aligned to the concatenated genome of the two species and peaks were called in intergenic regions using MACS2 (). Instances of allelic bias were detected using the same methods used for identifying ASE ( Materials and Methods ). ( C and D ) ChIP-seq of Tye7 in the interspecies hybrid shows differential binding to promoters of glycolysis genes PFK1 and HXK2 , respectively. The boxes depict the 1,000 base pair regions of the intergenic region upstream of the glycolysis genes in C. albicans ( Left ) and C. dubliniensis ( Right ), centered around the called ChIP peak. Darker- and lighter-colored lines represent ChIP-seq reads obtained from two replicate experiments in the interspecies hybrid. Magenta lines depict reads that map to the C. albicans genome, whereas green lines depict reads that map to the C. dubliniensis genome. Gray