Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 626801 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#626801, RRID:AB_2235686
- Product name
- Purified anti-c-Myc
- Antibody type
- Monoclonal
- Antigen
- amino acids 408-439, C-terminal region of human c-myc
- Description
- The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. c-myc is part of a heterodimeric complex with MAX that acts as a potent transcriptional activator. c-myc is modified by glycosylation and phosphorylation and has been shown to interact with a number of proteins including SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, and Sp1. c-myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that the observed protein size is approximately 41 kD. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The 9E10 antibody has been shown to be useful in a number of applications including Western blotting, direct ELISA, flow cytometry, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and immunoaffinity purification of proteins expressing the human c-myc tag.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
Submitted references Autoregulation of MARCH1 expression by dimerization and autoubiquitination.
Human and mouse ZFY genes produce a conserved testis-specific transcript encoding a zinc finger protein with a short acidic domain and modified transactivation potential.
Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
Bourgeois-Daigneault MC, Thibodeau J
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):4959-70
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):4959-70
Human and mouse ZFY genes produce a conserved testis-specific transcript encoding a zinc finger protein with a short acidic domain and modified transactivation potential.
Decarpentrie F, Vernet N, Mahadevaiah SK, Longepied G, Streichemberger E, Aknin-Seifer I, Ojarikre OA, Burgoyne PS, Metzler-Guillemain C, Mitchell MJ
Human molecular genetics 2012 Jun 15;21(12):2631-45
Human molecular genetics 2012 Jun 15;21(12):2631-45
Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
Kondo S, Wakisaka N, Muramatsu M, Zen Y, Endo K, Murono S, Sugimoto H, Yamaoka S, Pagano JS, Yoshizaki T
Journal of virology 2011 Nov;85(21):11255-64
Journal of virology 2011 Nov;85(21):11255-64
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Jurkat extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-c-myc monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Intact (non-degraded) c-myc with a molecular weight of approximately 62 kD is detected in this Western blot experiment.
- Conjugate
- Unconjugated