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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-16879 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-WT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16887 targets eNOS in immunohistochemistry (paraffin) applications and shows reactivity with Bovine, Canine, Human, mouse, Porcine, and Rat samples. Suggested positive control is capillary endothelium and regenerative endothelium. The PA5-16887 immunogen is a synthetic peptide derived from C-ternimal of human eNOS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Diagnostic pitfalls of differentiating desmoplastic small round cell tumor (DSRCT) from Wilms tumor (WT): overlapping morphologic and immunohistochemical features.
Intrauterine growth restriction leads to a dysregulation of Wilms' tumour supressor gene 1 (WT1) and to early podocyte alterations.
Proliferation and maturation of microvessels in arteriovenous malformations--expression patterns of angiogenic and cell cycle-dependent factors.
Connexin 43 regulates epicardial cell polarity and migration in coronary vascular development.
Arnold MA, Schoenfield L, Limketkai BN, Arnold CA
The American journal of surgical pathology 2014 Sep;38(9):1220-6
The American journal of surgical pathology 2014 Sep;38(9):1220-6
Intrauterine growth restriction leads to a dysregulation of Wilms' tumour supressor gene 1 (WT1) and to early podocyte alterations.
Menendez-Castro C, Hilgers KF, Amann K, Daniel C, Cordasic N, Wachtveitl R, Fahlbusch F, Plank C, Dötsch J, Rascher W, Hartner A
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2013 Jun;28(6):1407-17
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2013 Jun;28(6):1407-17
Proliferation and maturation of microvessels in arteriovenous malformations--expression patterns of angiogenic and cell cycle-dependent factors.
Meijer-Jorna LB, van der Loos CM, Teeling P, de Boer OJ, Florquin S, van der Horst CM, van der Wal AC
Journal of cutaneous pathology 2012 Jun;39(6):610-20
Journal of cutaneous pathology 2012 Jun;39(6):610-20
Connexin 43 regulates epicardial cell polarity and migration in coronary vascular development.
Rhee DY, Zhao XQ, Francis RJ, Huang GY, Mably JD, Lo CW
Development (Cambridge, England) 2009 Sep;136(18):3185-93
Development (Cambridge, England) 2009 Sep;136(18):3185-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of WT1 was performed using 70% confluent log phase SW-480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with WT1 Rabbit Polyclonal Antibody (Product # PA5-16879) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
