orb197802

antibody from Biorbyt

Targeting: WT1 AWT1, GUD, NPHS4, WAGR, WIT-2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

orb197802

Provider

Biorbyt

Product name

WT1 antibody

Antibody type

Monoclonal

Description

Mouse monoclonal antibody to WT1

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5G11A5

Vial size

100 μg

Storage

Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C

Western blot

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using WT1 antibody

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of human WT1 using WT1 antibody

ELISA

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of WT1 antibody

Flow cytometry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells lysates using WT1 antibody