H00007490-M02

antibody from Abnova Corporation

Targeting: WT1 AWT1, GUD, NPHS4, WAGR, WIT-2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007490-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007490-M02, RRID:AB_875934

Product name

WT1 monoclonal antibody (M02), clone 3B12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant WT1.

Antigen sequence

QDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNK
RYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRS
DQLKRHQRRHTGVKPFQCKTC


Isotype

IgG

Antibody clone number

3B12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of WT1 expression in transfected 293T cell line by WT1 monoclonal antibody (M02), clone 3B12.Lane 1: WT1 transfected lysate(34.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged WT1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol