MA1-163

antibody from Invitrogen Antibodies

Targeting: CCR7 BLR2, CD197, CDw197, CMKBR7, EBI1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-163

Provider

Invitrogen Antibodies

Product name

CCR7 Monoclonal Antibody (4B12)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes.

Antibody clone number

4B12

Concentration

1 mg/mL

References

Submitted references

miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity.

Serr I, Fürst RW, Ott VB, Scherm MG, Nikolaev A, Gökmen F, Kälin S, Zillmer S, Bunk M, Weigmann B, Kunschke N, Loretz B, Lehr CM, Kirchner B, Haase B, Pfaffl M, Waisman A, Willis RA, Ziegler AG, Daniel C
Proceedings of the National Academy of Sciences of the United States of America 2016 Oct 25;113(43):E6659-E6668

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CCR7 was performed by loading the indicated amounts of Jurkat whole cell lysate and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% nonfat dry milk in TBST for at least 1 hour at room temperature. CCR7 was detected at ~43 kD using a CCR7 monoclonal antibody (Product # MA1-163) at a dilution of 1:500 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rat IgG secondary antibody (Product # 31470) at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CCR7 on primary mouse splenocytes. Cells were blocked with an anti-CD16/32 monoclonal antibody (Fc receptor blocker) for 30 minutes on ice, and then stained with a CCR7 monoclonal antibody (Product # MA1-163), using 5 µg of antibody per 1x106 cells, in 1X PBS containing 5% FCS. After incubation of the primary antibody for 1 hour at 37C, the cells were stained with a FITC-conjugated mouse anti-rat IgG2a secondary antibody (Product # SA1-25265) for at least 30 minutes on ice. A representative 20,000 cells were acquired for each sample, and the appropriate FSC vs SSC gate (left panel) was used to assess CCR7 staining on lymphocytes (right panel). The cells were also stained with a PE-conjugated hamster anti-mouse CD3 epsilon monoclonal antibody.