17-1979-41

CCR7 antibody from Invitrogen Antibodies
BLR2, CD197, CDw197, CMKBR7, EBI1

Antibody data

Product number
17-1979-41
Provider
Invitrogen Antibodies
Product name
Anti-CD197 (CCR7) Monoclonal Antibody (3D12), APC, eBioscience™
Provider product page
Invitrogen Antibodies - 17-1979-41
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding. Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Rat
Isotype
IgG
Antibody clone number
3D12
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
Provider Type Product Number
- No reagents -