200-301-065s

antibody from Rockland Immunochemicals, Inc.

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-065s

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-065s, RRID:AB_10924419

Product name

Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065S

Antibody type

Monoclonal

Vial size

25 µl

References

Submitted references

Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-derived Stem Cells.

Windmöller BA, Höving AL, Knabbe C, Greiner JFW
Stem cell reviews and reports 2022 Apr;18(4):1510-1520

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Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols.

Chansard A, Dubrulle N, Poujol de Molliens M, Falanga PB, Stephen T, Hasan M, van Zandbergen G, Aulner N, Shorte SL, David-Watine B
Frontiers in immunology 2020;11:569331

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A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice.

Ryu S, Han HM, Song PI, Armstrong CA, Park Y
PloS one 2015;10(7):e0132619

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rockland monoclonal anti p65 200-301-065 was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti mouse antibody at a dilution of 1:10000. Image provided courtesy of Dr. Al Baldwin, University of North Carolina, Chapel Hill.

Validation comment

Western Blot