11222

antibody from Signalway Antibody LLC

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11222

Provider

Signalway Antibody LLC

Product name

4E-BP1(Phospho-Thr36) Antibody

Antibody type

Polyclonal

Antigen

The antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 36.

Host

Rabbit

Conjugate

Unconjugated

Vial size

50µl

Western blot

Supportive validation

Submitted by

Signalway Antibody LLC (provider)

Main image

Experimental details

Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (#11222, Lane 3 and 4).

Immunocytochemistry

Supportive validation

Submitted by

Signalway Antibody LLC (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B antibody at 1: 200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Signalway Antibody LLC (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody (#11222).