Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-17728 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 219 µg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HEK-293 treated with EGF (200 ng/mL for 10 min) (Lane 2) and HEK-293 treated with Rapamycin (20 nM for 30 min) followed by EGF (200 ng/mL for 10 min) (Lane 3). The blot was probed with Anti-Phospho-4EBP1 (Thr37, Thr46) Rabbit Polyclonal Antibody (Product PA5-17728 #, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Phospho-4EBP1 (Thr37, Thr46) was detected and showed increase in phosphorylation on EGF treatment and decrease upon rapamycin treatment.