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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [46]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB200-157 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-157, RRID:AB_10002070
- Product name
- Rabbit Polyclonal 4EBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Delayed recovery of skeletal muscle mass following hindlimb immobilization in mTOR heterozygous mice.
A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells.
Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication.
Hyperoxia inhibits protein synthesis and increases eIF2α phosphorylation in the newborn rat lung.
Phosphatidic acid mediates activation of mTORC1 through the ERK signaling pathway.
Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation.
Activation of the mammalian target of rapamycin (mTOR) is essential for oligodendrocyte differentiation.
Age-related deficit in load-induced skeletal muscle growth.
The leucine content of a complete meal directs peak activation but not duration of skeletal muscle protein synthesis and mammalian target of rapamycin signaling in rats.
Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver.
Skeletal and cardiac myopathy in HIV-1 transgenic rats.
Fed levels of amino acids are required for the somatotropin-induced increase in muscle protein synthesis.
Activation of AMP-activated protein kinase by 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside prevents leucine-stimulated protein synthesis in rat skeletal muscle.
Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin.
Rapid turnover of the mTOR complex 1 (mTORC1) repressor REDD1 and activation of mTORC1 signaling following inhibition of protein synthesis.
Eukaryotic translation initiation factor 4F architectural alterations accompany translation initiation factor redistribution in poxvirus-infected cells.
Amino acids augment muscle protein synthesis in neonatal pigs during acute endotoxemia by stimulating mTOR-dependent translation initiation.
Retrograde nuclear accumulation of cytoplasmic tRNA in rat hepatoma cells in response to amino acid deprivation.
Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication.
Cell size and invasion in TGF-beta-induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway.
Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption.
Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation.
Feeding meals containing soy or whey protein after exercise stimulates protein synthesis and translation initiation in the skeletal muscle of male rats.
Activation by insulin and amino acids of signaling components leading to translation initiation in skeletal muscle of neonatal pigs is developmentally regulated.
Glucose stimulates protein synthesis in skeletal muscle of neonatal pigs through an AMPK- and mTOR-independent process.
Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle.
Leucine in food mediates some of the postprandial rise in plasma leptin concentrations.
Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts.
AKT-independent phosphorylation of TSC2 and activation of mTOR and ribosomal protein S6 kinase signaling by prostaglandin F2alpha.
The increase in human muscle protein synthesis induced by food intake is similar when assessed with the constant infusion and flooding techniques.
Time course changes in signaling pathways and protein synthesis in C2C12 myotubes following AMPK activation by AICAR.
IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis.
Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs.
Cellular energy status modulates translational control mechanisms in ischemic-reperfused rat hearts.
Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle.
Repression of protein synthesis and mTOR signaling in rat liver mediated by the AMPK activator aminoimidazole carboxamide ribonucleoside.
Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation.
Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.
Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture.
Acute treatment with TNF-alpha attenuates insulin-stimulated protein synthesis in cultures of C2C12 myotubes through a MEK1-sensitive mechanism.
Hindlimb casting decreases muscle mass in part by proteasome-dependent proteolysis but independent of protein synthesis.
Differential effect of sepsis on ability of leucine and IGF-I to stimulate muscle translation initiation.
A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1.
A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1.
Preservation of liver protein synthesis during dietary leucine deprivation occurs at the expense of skeletal muscle mass in mice deleted for eIF2 kinase GCN2.
Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle.
Lang SM, Kazi AA, Hong-Brown L, Lang CH
PloS one 2012;7(6):e38910
PloS one 2012;7(6):e38910
A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells.
Jozefczuk J, Kashofer K, Ummanni R, Henjes F, Rehman S, Geenen S, Wruck W, Regenbrecht C, Daskalaki A, Wierling C, Turano P, Bertini I, Korf U, Zatloukal K, Westerhoff HV, Lehrach H, Adjaye J
Frontiers in physiology 2012;3:339
Frontiers in physiology 2012;3:339
Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication.
Kobayashi M, Arias C, Garabedian A, Palmenberg AC, Mohr I
Journal of virology 2012 Oct;86(19):10686-94
Journal of virology 2012 Oct;86(19):10686-94
Hyperoxia inhibits protein synthesis and increases eIF2α phosphorylation in the newborn rat lung.
Konsavage W, Zhang L, Vary T, Shenberger JS
American journal of physiology. Lung cellular and molecular physiology 2010 May;298(5):L678-86
American journal of physiology. Lung cellular and molecular physiology 2010 May;298(5):L678-86
Phosphatidic acid mediates activation of mTORC1 through the ERK signaling pathway.
Winter JN, Fox TE, Kester M, Jefferson LS, Kimball SR
American journal of physiology. Cell physiology 2010 Aug;299(2):C335-44
American journal of physiology. Cell physiology 2010 Aug;299(2):C335-44
Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation.
Wilson FA, Suryawan A, Orellana RA, Kimball SR, Gazzaneo MC, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2009 Oct;139(10):1873-80
The Journal of nutrition 2009 Oct;139(10):1873-80
Activation of the mammalian target of rapamycin (mTOR) is essential for oligodendrocyte differentiation.
Tyler WA, Gangoli N, Gokina P, Kim HA, Covey M, Levison SW, Wood TL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 13;29(19):6367-78
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 13;29(19):6367-78
Age-related deficit in load-induced skeletal muscle growth.
Hwee DT, Bodine SC
The journals of gerontology. Series A, Biological sciences and medical sciences 2009 Jun;64(6):618-28
The journals of gerontology. Series A, Biological sciences and medical sciences 2009 Jun;64(6):618-28
The leucine content of a complete meal directs peak activation but not duration of skeletal muscle protein synthesis and mammalian target of rapamycin signaling in rats.
Norton LE, Layman DK, Bunpo P, Anthony TG, Brana DV, Garlick PJ
The Journal of nutrition 2009 Jun;139(6):1103-9
The Journal of nutrition 2009 Jun;139(6):1103-9
Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver.
Baum JI, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E410-5
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E410-5
Skeletal and cardiac myopathy in HIV-1 transgenic rats.
Pruznak AM, Hong-Brown L, Lantry R, She P, Frost RA, Vary TC, Lang CH
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E964-73
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E964-73
Fed levels of amino acids are required for the somatotropin-induced increase in muscle protein synthesis.
Wilson FA, Suryawan A, Orellana RA, Nguyen HV, Jeyapalan AS, Gazzaneo MC, Davis TA
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E876-83
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E876-83
Activation of AMP-activated protein kinase by 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside prevents leucine-stimulated protein synthesis in rat skeletal muscle.
Pruznak AM, Kazi AA, Frost RA, Vary TC, Lang CH
The Journal of nutrition 2008 Oct;138(10):1887-94
The Journal of nutrition 2008 Oct;138(10):1887-94
Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin.
Wilson FA, Orellana RA, Suryawan A, Nguyen HV, Jeyapalan AS, Frank J, Davis TA
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E187-94
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E187-94
Rapid turnover of the mTOR complex 1 (mTORC1) repressor REDD1 and activation of mTORC1 signaling following inhibition of protein synthesis.
Kimball SR, Do AN, Kutzler L, Cavener DR, Jefferson LS
The Journal of biological chemistry 2008 Feb 8;283(6):3465-75
The Journal of biological chemistry 2008 Feb 8;283(6):3465-75
Eukaryotic translation initiation factor 4F architectural alterations accompany translation initiation factor redistribution in poxvirus-infected cells.
Walsh D, Arias C, Perez C, Halladin D, Escandon M, Ueda T, Watanabe-Fukunaga R, Fukunaga R, Mohr I
Molecular and cellular biology 2008 Apr;28(8):2648-58
Molecular and cellular biology 2008 Apr;28(8):2648-58
Amino acids augment muscle protein synthesis in neonatal pigs during acute endotoxemia by stimulating mTOR-dependent translation initiation.
Orellana RA, Jeyapalan A, Escobar J, Frank JW, Nguyen HV, Suryawan A, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Nov;293(5):E1416-25
American journal of physiology. Endocrinology and metabolism 2007 Nov;293(5):E1416-25
Retrograde nuclear accumulation of cytoplasmic tRNA in rat hepatoma cells in response to amino acid deprivation.
Shaheen HH, Horetsky RL, Kimball SR, Murthi A, Jefferson LS, Hopper AK
Proceedings of the National Academy of Sciences of the United States of America 2007 May 22;104(21):8845-50
Proceedings of the National Academy of Sciences of the United States of America 2007 May 22;104(21):8845-50
Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication.
Lang CH, Frost RA, Vary TC
American journal of physiology. Endocrinology and metabolism 2007 Jun;292(6):E1497-506
American journal of physiology. Endocrinology and metabolism 2007 Jun;292(6):E1497-506
Cell size and invasion in TGF-beta-induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway.
Lamouille S, Derynck R
The Journal of cell biology 2007 Jul 30;178(3):437-51
The Journal of cell biology 2007 Jul 30;178(3):437-51
Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption.
Vary TC, Kimball SR, Sumner A
American journal of physiology. Regulatory, integrative and comparative physiology 2007 Feb;292(2):R778-87
American journal of physiology. Regulatory, integrative and comparative physiology 2007 Feb;292(2):R778-87
Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation.
Orellana RA, Kimball SR, Suryawan A, Escobar J, Nguyen HV, Jefferson LS, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Feb;292(2):E629-36
American journal of physiology. Endocrinology and metabolism 2007 Feb;292(2):E629-36
Feeding meals containing soy or whey protein after exercise stimulates protein synthesis and translation initiation in the skeletal muscle of male rats.
Anthony TG, McDaniel BJ, Knoll P, Bunpo P, Paul GL, McNurlan MA
The Journal of nutrition 2007 Feb;137(2):357-62
The Journal of nutrition 2007 Feb;137(2):357-62
Activation by insulin and amino acids of signaling components leading to translation initiation in skeletal muscle of neonatal pigs is developmentally regulated.
Suryawan A, Orellana RA, Nguyen HV, Jeyapalan AS, Fleming JR, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1597-605
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1597-605
Glucose stimulates protein synthesis in skeletal muscle of neonatal pigs through an AMPK- and mTOR-independent process.
Jeyapalan AS, Orellana RA, Suryawan A, O'Connor PM, Nguyen HV, Escobar J, Frank JW, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Aug;293(2):E595-603
American journal of physiology. Endocrinology and metabolism 2007 Aug;293(2):E595-603
Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle.
Lang CH
American journal of physiology. Endocrinology and metabolism 2006 Sep;291(3):E666-74
American journal of physiology. Endocrinology and metabolism 2006 Sep;291(3):E666-74
Leucine in food mediates some of the postprandial rise in plasma leptin concentrations.
Lynch CJ, Gern B, Lloyd C, Hutson SM, Eicher R, Vary TC
American journal of physiology. Endocrinology and metabolism 2006 Sep;291(3):E621-30
American journal of physiology. Endocrinology and metabolism 2006 Sep;291(3):E621-30
Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts.
Vary TC, Lynch CJ
The Journal of nutrition 2006 Sep;136(9):2284-90
The Journal of nutrition 2006 Sep;136(9):2284-90
AKT-independent phosphorylation of TSC2 and activation of mTOR and ribosomal protein S6 kinase signaling by prostaglandin F2alpha.
Arvisais EW, Romanelli A, Hou X, Davis JS
The Journal of biological chemistry 2006 Sep 15;281(37):26904-13
The Journal of biological chemistry 2006 Sep 15;281(37):26904-13
The increase in human muscle protein synthesis induced by food intake is similar when assessed with the constant infusion and flooding techniques.
Caso G, Garlick PJ, Ballou LM, Vosswinkel JA, Gelato MC, McNurlan MA
The Journal of nutrition 2006 Jun;136(6):1504-10
The Journal of nutrition 2006 Jun;136(6):1504-10
Time course changes in signaling pathways and protein synthesis in C2C12 myotubes following AMPK activation by AICAR.
Williamson DL, Bolster DR, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism 2006 Jul;291(1):E80-9
American journal of physiology. Endocrinology and metabolism 2006 Jul;291(1):E80-9
IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis.
Vary TC
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Feb;290(2):R313-21
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Feb;290(2):R313-21
Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs.
Escobar J, Frank JW, Suryawan A, Nguyen HV, Kimball SR, Jefferson LS, Davis TA
American journal of physiology. Endocrinology and metabolism 2006 Apr;290(4):E612-21
American journal of physiology. Endocrinology and metabolism 2006 Apr;290(4):E612-21
Cellular energy status modulates translational control mechanisms in ischemic-reperfused rat hearts.
Crozier SJ, Vary TC, Kimball SR, Jefferson LS
American journal of physiology. Heart and circulatory physiology 2005 Sep;289(3):H1242-50
American journal of physiology. Heart and circulatory physiology 2005 Sep;289(3):H1242-50
Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle.
Hong-Brown LQ, Pruznak AM, Frost RA, Vary TC, Lang CH
American journal of physiology. Endocrinology and metabolism 2005 Sep;289(3):E382-90
American journal of physiology. Endocrinology and metabolism 2005 Sep;289(3):E382-90
Repression of protein synthesis and mTOR signaling in rat liver mediated by the AMPK activator aminoimidazole carboxamide ribonucleoside.
Reiter AK, Bolster DR, Crozier SJ, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E980-8
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E980-8
Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation.
Escobar J, Frank JW, Suryawan A, Nguyen HV, Kimball SR, Jefferson LS, Davis TA
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E914-21
American journal of physiology. Endocrinology and metabolism 2005 May;288(5):E914-21
Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.
Kubica N, Bolster DR, Farrell PA, Kimball SR, Jefferson LS
The Journal of biological chemistry 2005 Mar 4;280(9):7570-80
The Journal of biological chemistry 2005 Mar 4;280(9):7570-80
Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture.
Dufner DA, Bederman IR, Brunengraber DZ, Rachdaoui N, Ismail-Beigi F, Siegfried BA, Kimball SR, Previs SF
American journal of physiology. Endocrinology and metabolism 2005 Jun;288(6):E1277-83
American journal of physiology. Endocrinology and metabolism 2005 Jun;288(6):E1277-83
Acute treatment with TNF-alpha attenuates insulin-stimulated protein synthesis in cultures of C2C12 myotubes through a MEK1-sensitive mechanism.
Williamson DL, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism 2005 Jul;289(1):E95-104
American journal of physiology. Endocrinology and metabolism 2005 Jul;289(1):E95-104
Hindlimb casting decreases muscle mass in part by proteasome-dependent proteolysis but independent of protein synthesis.
Krawiec BJ, Frost RA, Vary TC, Jefferson LS, Lang CH
American journal of physiology. Endocrinology and metabolism 2005 Dec;289(6):E969-80
American journal of physiology. Endocrinology and metabolism 2005 Dec;289(6):E969-80
Differential effect of sepsis on ability of leucine and IGF-I to stimulate muscle translation initiation.
Lang CH, Frost RA
American journal of physiology. Endocrinology and metabolism 2004 Oct;287(4):E721-30
American journal of physiology. Endocrinology and metabolism 2004 Oct;287(4):E721-30
A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1.
Kimball SR, Horetsky RL, Jefferson LS
Analytical biochemistry 2004 Feb 15;325(2):364-8
Analytical biochemistry 2004 Feb 15;325(2):364-8
A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1.
Kimball SR, Horetsky RL, Jefferson LS
Analytical biochemistry 2004 Feb 15;325(2):364-8
Analytical biochemistry 2004 Feb 15;325(2):364-8
Preservation of liver protein synthesis during dietary leucine deprivation occurs at the expense of skeletal muscle mass in mice deleted for eIF2 kinase GCN2.
Anthony TG, McDaniel BJ, Byerley RL, McGrath BC, Cavener DR, McNurlan MA, Wek RC
The Journal of biological chemistry 2004 Aug 27;279(35):36553-61
The Journal of biological chemistry 2004 Aug 27;279(35):36553-61
Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle.
Lang CH, Frost RA, Deshpande N, Kumar V, Vary TC, Jefferson LS, Kimball SR
American journal of physiology. Endocrinology and metabolism 2003 Dec;285(6):E1205-15
American journal of physiology. Endocrinology and metabolism 2003 Dec;285(6):E1205-15
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
