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- References [23]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 60246-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- 4EBP1 antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated 4EBP1 antibody (Cat. #60246-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, rat, pig, mouse and has been validated for the following applications: IF, WB,ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2C3F3
- Vial size
- 20ul, 150ul
Submitted references Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening.
UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.
TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.
New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects.
p53-R273H Sustains ROS, Pro-Inflammatory Cytokine Release and mTOR Activation While Reducing Autophagy, Mitophagy and UCP2 Expression, Effects Prevented by wtp53.
Mammalian target of rapamycin signaling pathway is involved in synaptic plasticity of the spinal dorsal horn and neuropathic pain in rats by regulating autophagy.
STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.
The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
miR-196b-5p controls adipocyte differentiation and lipogenesis through regulating mTORC1 and TGF-β signaling.
Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.
Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors.
A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo.
Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.
Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.
FGFR2 Promotes Gastric Cancer Progression by Inhibiting the Expression of Thrombospondin4 via PI3K-Akt-Mtor Pathway.
Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling.
Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.
Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling.
EPS8 regulates proliferation, apoptosis and chemosensitivity in BCR-ABL positive cells via the BCR-ABL/PI3K/AKT/mTOR pathway.
MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer.
Cucurbitacin B induces autophagy and apoptosis by suppressing CIP2A/PP2A/mTORC1 signaling axis in human cisplatin resistant gastric cancer cells.
Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.
Liao W, Yang W, Xu J, Yan Z, Pan M, Xu X, Zhou S, Zhu Y, Lan J, Zeng M, Han X, Li S, Li Y, Liang K, Gao Y, Peng Q
Frontiers in pharmacology 2021;12:658197
Frontiers in pharmacology 2021;12:658197
UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma.
Zhang M, Chen H, Qin P, Cai T, Li L, Chen R, Liu S, Chen H, Lin W, Chen H, Strickland AL, Xiong H, Jiang Q
Aging 2021 Sep 14;13(17):21202-21215
Aging 2021 Sep 14;13(17):21202-21215
TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.
Zhou C, Liang Y, Zhou L, Yan Y, Liu N, Zhang R, Huang Y, Wang M, Tang Y, Ali DW, Wang Y, Michalak M, Chen XZ, Tang J
Autophagy 2021 Oct;17(10):3175-3195
Autophagy 2021 Oct;17(10):3175-3195
New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects.
Arena A, Romeo MA, Benedetti R, Masuelli L, Bei R, Gilardini Montani MS, Cirone M
Pharmaceuticals (Basel, Switzerland) 2021 Oct 22;14(11)
Pharmaceuticals (Basel, Switzerland) 2021 Oct 22;14(11)
p53-R273H Sustains ROS, Pro-Inflammatory Cytokine Release and mTOR Activation While Reducing Autophagy, Mitophagy and UCP2 Expression, Effects Prevented by wtp53.
Romeo MA, Gilardini Montani MS, Benedetti R, Arena A, D'Orazi G, Cirone M
Biomolecules 2021 Feb 24;11(3)
Biomolecules 2021 Feb 24;11(3)
Mammalian target of rapamycin signaling pathway is involved in synaptic plasticity of the spinal dorsal horn and neuropathic pain in rats by regulating autophagy.
Hu J, Chen X, Cheng J, Kong F, Xia H, Wu J
Neuroreport 2021 Aug 4;32(11):925-935
Neuroreport 2021 Aug 4;32(11):925-935
STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.
Zhou C, Qian X, Hu M, Zhang R, Liu N, Huang Y, Yang J, Zhang J, Bai H, Yang Y, Wang Y, Ali D, Michalak M, Chen XZ, Tang J
Autophagy 2020 Oct;16(10):1786-1806
Autophagy 2020 Oct;16(10):1786-1806
The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.
Chen LY, Wang L, Ren YX, Pang Z, Liu Y, Sun XD, Tu J, Zhi Z, Qin Y, Sun LN, Li JM
Molecular cancer 2020 Nov 23;19(1):164
Molecular cancer 2020 Nov 23;19(1):164
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
Dai W, Xu L, Yu X, Zhang G, Guo H, Liu H, Song G, Weng S, Dong L, Zhu J, Liu T, Guo C, Shen X
Journal of hepatology 2020 May;72(5):909-923
Journal of hepatology 2020 May;72(5):909-923
miR-196b-5p controls adipocyte differentiation and lipogenesis through regulating mTORC1 and TGF-β signaling.
Shi Y, Li F, Wang S, Wang C, Xie Y, Zhou J, Li X, Wang B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jul;34(7):9207-9222
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jul;34(7):9207-9222
Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.
Lu XY, Shi XJ, Hu A, Wang JQ, Ding Y, Jiang W, Sun M, Zhao X, Luo J, Qi W, Song BL
Nature 2020 Dec;588(7838):479-484
Nature 2020 Dec;588(7838):479-484
Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors.
Chen Y, Gao H, Liu Q, Xie W, Li B, Cheng S, Guo J, Fang Q, Zhu H, Wang Z, Wang J, Li C, Zhang Y
Aging 2020 Dec 29;13(1):1422-1439
Aging 2020 Dec 29;13(1):1422-1439
A CRISPR knockout negative screen reveals synergy between CDKs inhibitor and metformin in the treatment of human cancer in vitro and in vivo.
Ma Y, Zhu Q, Liang J, Li Y, Li M, Zhang Y, Wang X, Zeng Y, Jiao Y
Signal transduction and targeted therapy 2020 Aug 19;5(1):152
Signal transduction and targeted therapy 2020 Aug 19;5(1):152
Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.
Zheng R, Huang S, Zhu J, Lin W, Xu H, Zheng X
Cell and tissue research 2019 Oct;378(1):113-125
Cell and tissue research 2019 Oct;378(1):113-125
Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.
Zhou C, Wang M, Yang J, Xiong H, Wang Y, Tang J
Cell communication and signaling : CCS 2019 Aug 22;17(1):105
Cell communication and signaling : CCS 2019 Aug 22;17(1):105
FGFR2 Promotes Gastric Cancer Progression by Inhibiting the Expression of Thrombospondin4 via PI3K-Akt-Mtor Pathway.
Huang T, Liu D, Wang Y, Li P, Sun L, Xiong H, Dai Y, Zou M, Yuan X, Qiu H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(4):1332-1345
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(4):1332-1345
Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling.
Jin J, Hu K, Ye M, Wu D, He Q
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(2):765-772
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(2):765-772
Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.
Yang Y, Qi Q, Wang Y, Shi Y, Yang W, Cen Y, Zhu E, Li X, Chen D, Wang B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jun;32(6):3096-3107
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jun;32(6):3096-3107
Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling.
Usman MW, Gao J, Zheng T, Rui C, Li T, Bian X, Cheng H, Liu P, Luo F
Cell death & disease 2018 Jul 24;9(8):809
Cell death & disease 2018 Jul 24;9(8):809
EPS8 regulates proliferation, apoptosis and chemosensitivity in BCR-ABL positive cells via the BCR-ABL/PI3K/AKT/mTOR pathway.
Huang R, Liu H, Chen Y, He Y, Kang Q, Tu S, He Y, Zhou X, Wang L, Yang J, Wu A, Li Y
Oncology reports 2018 Jan;39(1):119-128
Oncology reports 2018 Jan;39(1):119-128
MAN1B1 is associated with poor prognosis and modulates proliferation and apoptosis in bladder cancer.
Wang HF, Wu JH, Gai JW, Yang SQ, Ma QT, Ma HS, Feng Q
Gene 2018 Dec 30;679:314-319
Gene 2018 Dec 30;679:314-319
Cucurbitacin B induces autophagy and apoptosis by suppressing CIP2A/PP2A/mTORC1 signaling axis in human cisplatin resistant gastric cancer cells.
Liu X, Duan C, Ji J, Zhang T, Yuan X, Zhang Y, Ma W, Yang J, Yang L, Jiang Z, Yu H, Liu Y
Oncology reports 2017 Jul;38(1):271-278
Oncology reports 2017 Jul;38(1):271-278
Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.
Wang D, Wang M, Jiang N, Zhang Y, Bian X, Wang X, Roberts TM, Zhao JJ, Liu P, Cheng H
Oncotarget 2016 Mar 15;7(11):13153-66
Oncotarget 2016 Mar 15;7(11):13153-66
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig(4EBP1 antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The 4EBP1 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human 4EBP1. This antibody recognizes human,mouse antigen. The 4EBP1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
