Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP11125PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11125PU-N, RRID:AB_1753503
- Product name
- anti HDAC3 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-term region of human HDAC3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (Left) The anti-HDAC3 Pab (Cat.#AP11125PU-N) is used in Western blot to detect HDAC3 in mouse brain tissue lysate. (Right) Western blot analysis of HDAC3 (arrow) using rabbit polyclonal HDAC3 Antibody (M1) (Cat.#AP11125PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC3 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HDAC3 antibody (N-term) (Cat.#AP11125PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.