Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-24861 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4D11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP Citrate Lyase in Lane 1: the kidney tissue lysate of mouse, Lane 2: the colon tissue lysate of mouse, Lane 2: MCF7 whole cell lysate. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) at a dilution of 1:1,000.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP Citrate Lyase in Lane 1: the kidney tissue lysate of mouse, Lane 2: the colon tissue lysate of mouse, Lane 2: MCF7 whole cell lysate. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ATP Citrate Lyase was achieved by transfecting A549 with ATP Citrate Lyase specific siRNAs (Silencer® select Product # S915, S916). Western blot analysis (Fig. a) was performed using Whole cell extracts from the ATP Citrate Lyase untransfected cells (lane 1), non-targeting scrambled siRNA transfected cells (lane 2) and knockdown cells (lane 3).The blot was probed with ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11) (Product # MA5-24861, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP Citrate Lyase.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11)(Product # MA5-24861) and a 120kDa band corresponding to ATP Citrate Lyase was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), PC-3 (Lane 3), A549 (Lane 4), SK-O-V3 (Lane 5), Mouse Kidney (Lane 6), Rat Kidney (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP Citrate Lyase in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP Citrate Lyase in CRC cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP Citrate Lyase in A549 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861).
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ATP Citrate Lyase was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 0.2% BSA for 45 minutes at room temperature. The cells were labeled with ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4D11) (Product # MA5-24861) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
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- Experimental details
- Immunohistochemistry analysis of ATP Citrate Lyase in paraffin-embedded mouse thyroid tissue. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) followed by hematoxylin.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP Citrate Lyase in paraffin-embedded mouse kidney tissue. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) followed by hematoxylin.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry analysis of ATP Citrate Lyase in paraffin-embedded human thyroid tissue. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) followed by hematoxylin.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP Citrate Lyase in paraffin-embedded human breast carcinoma tissue. Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) followed by hematoxylin.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ATP Citrate Lyase in HeLa cells (blue) and compared with an unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with ATP Citrate Lyase monoclonal antibody (Product # MA5-24861) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.