PA5-21434

antibody from Invitrogen Antibodies

Targeting: BAG5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21434

Provider

Invitrogen Antibodies

Product name

BAG5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, Molt-4, Raji, mouse liver.

Concentration

0.68 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BAG5 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BAG5 Polyclonal Antibody (Product # PA5-21434) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BAG5 Polyclonal Antibody (Product # PA5-21434). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Molt-4. 10% SDS PAGE. BAG5 Polyclonal Antibody (Product # PA5-21434) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BAG5 in paraformaldehyde-fixed HeLa cells using a BAG5 polyclonal antibody (Product # PA5-21434) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5 (Product # PA5-21434) antibody at 1:200 dilution.