Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX104124 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104124, RRID:AB_1950640
- Product name
- Cytokeratin 14 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references Knock-down of filaggrin influences the mitogen-activated protein kinases signaling pathway in normal human epidermal keratinocytes.
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Elucidating Mechanisms of Bladder Repair after Hyaluronan Instillation in Ketamine-Induced Ulcerative Cystitis in Animal Model.
Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Biological effect of ketamine in urothelial cell lines and global gene expression analysis in the bladders of ketamine‑injected mice.
Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland.
Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
Wang S, Qiu L, Meng X, Dang N
Medecine sciences : M/S 2018 Oct;34 Focus issue F1:94-98
Medecine sciences : M/S 2018 Oct;34 Focus issue F1:94-98
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Fan JR, Lee HT, Lee W, Lin CH, Hsu CY, Hsieh CH, Shyu WC
Cell death & disease 2018 May 1;9(5):502
Cell death & disease 2018 May 1;9(5):502
Elucidating Mechanisms of Bladder Repair after Hyaluronan Instillation in Ketamine-Induced Ulcerative Cystitis in Animal Model.
Lee YL, Lin KL, Chuang SM, Lee YC, Lu MC, Wu BN, Wu WJ, Yuan SF, Ho WT, Juan YS
The American journal of pathology 2017 Sep;187(9):1945-1959
The American journal of pathology 2017 Sep;187(9):1945-1959
Filaggrin silencing by shRNA directly impairs the skin barrier function of normal human epidermal keratinocytes and then induces an immune response.
Dang NN, Pang SG, Song HY, An LG, Ma XL
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2015 Jan;48(1):39-45
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2015 Jan;48(1):39-45
Knockdown of filaggrin influences the epidermal terminal differentiation via MAPK pathway in normal human epidermal keratinocytes.
Dang N, Pang S, Song H, Bian H, Zhang X, An L, Ma X
Molecular biology reports 2015 Feb;42(2):337-43
Molecular biology reports 2015 Feb;42(2):337-43
Biological effect of ketamine in urothelial cell lines and global gene expression analysis in the bladders of ketamine‑injected mice.
Shen CH, Wang ST, Lee YR, Liu SY, Li YZ, Wu JD, Chen YJ, Liu YW
Molecular medicine reports 2015 Feb;11(2):887-95
Molecular medicine reports 2015 Feb;11(2):887-95
Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells.
Igawa K, Kokubu C, Yusa K, Horie K, Yoshimura Y, Yamauchi K, Suemori H, Yokozeki H, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Katayama I, Takeda J
Stem cells translational medicine 2014 Sep;3(9):992-1001
Stem cells translational medicine 2014 Sep;3(9):992-1001
Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland.
Hassiotou F, Hepworth AR, Beltran AS, Mathews MM, Stuebe AM, Hartmann PE, Filgueira L, Blancafort P
Frontiers in oncology 2013;3:79
Frontiers in oncology 2013;3:79
Progesterone receptor A stability is mediated by glycogen synthase kinase-3β in the Brca1-deficient mammary gland.
Wang S, Li Y, Hsu PH, Lee SY, Kim Y, Lee EY
The Journal of biological chemistry 2013 Sep 6;288(36):26265-74
The Journal of biological chemistry 2013 Sep 6;288(36):26265-74
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cytokeratin 14 antibody detects KRT14 protein by Western blot analysis.A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract10 % SDS-PAGECytokeratin 14 antibody (GTX104124) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:HeLa S3(GTX14654)7.5% SDS PAGEGTX104124 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cytokeratin 14 antibody detects Cytokeratin 14 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody (GTX104124) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Lung CA, using Cytokeratin 14(GTX104124) antibody at 1:100 dilution.