MA5-11599

antibody from Invitrogen Antibodies

Targeting: KRT14 EBS3, EBS4

Provider product page for MA5-11599

Western blot

Antibody data

Antibody Data
Antigen structure
References [137]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]
Flow cytometry [1]
Other assay [14]

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Product number: MA5-11599 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cytokeratin 14 Monoclonal Antibody (LL002)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA5-11599 targets Cytokeratin 14 in IHC (P) and WB applications and shows reactivity with Human and Rat samples.
Antibody clone number: LL002
Concentration: 0.2 mg/mL

KRT14 protein structure - MA5-11599 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Loss of a2V leads to aberrant activation of Notch in luminal epithelial cells. Mammary glands of WT and KO mice were evaluated for distinct epithelial cell populations. ( a ) Immunofluorescent double staining for basal epithelial cells (CK14 - green) and luminal epithelial cells (CK18 - red) in pubertal mice. ( b ) Expression of NICD (red) costained basal epithelial marker (CK14 - green) in pubertal and pregnant mice by immunofluorescence reveals aberrant activation of Notch 1 in luminal epithelial cells (white arrows). Nucleus was stained with DAPI (diamidino-2-phenylindole; blue)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 The effect of Autism spectrum disorders (ASD) -related Streptococci on the polymeric immunoglobulin receptor (PIGR) protein level and the NF-kappaB signaling pathway in the human salivary gland (HSG) cells. (A) Cytokeratin 14 and PIGR expression in HSG cells was validated by immunofluorescent staining. Representative images of similar results are shown. (B) Control group: normal HSG cells; +BHI group: HSG cells treated with blank brain heart infusion broth; + S. mutans group: HSG cells co-incubated with heat-killed Streptococcus mutans . Western blotting was used to determine the protein levels of PIGR, NF-kappaB, and phosphorylated NF-kappaB (p-NF-kappaB). Representative pictures of western blots with similar results and the quantification of protein levels are shown. The above data are presented as the mean +- SEM, n = 3 per group, One-way ANOVA, * denotes p < 0.05 ** denotes p < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 8 MyD88-dependent signalling contributes to the development of skin pathology in OTULIN E-KO mice. a Kaplan-Meier plot depicting survival of mice of the indicated genotypes. b Graph depicting macroscopic skin score of mice of the indicated genotypes. The dots in the graphs represent individual mice. Mean +- s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Kruskal-Wallis test (one-sided). c Representative images from skin sections from 3-week-old mice of the indicated genotypes, stained with H&E ( n = 5) or immunostained with anti-CC3 ( n = 5), anti-CC8 ( n = 5) or anti-Ki-67 ( n = 5) antibodies. Scale bars: H&E = 100 mum; CC3, CC8, Ki-67 = 50 mum. d Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 ( n = 5), anti-K6 ( n = 5), anti-F4/80 ( n = 5) and anti-Gr-1 ( n = 5) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 mum; F4/80, Gr-1 = 30 mum. e Graphs depicting relative mRNA expression of the indicated genes in RNA from whole-skin tissue of 3-week-old mice of the indicated genotypes, measured by qRT-PCR. The dots in the graphs represent individual mice. Mean +- s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using Kruskal-Wallis test (one-sided). Control mice include littermates that do not express K14-Cre . Source data for a , b , e are provided as a Source data fi

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 1 Decreased enamel matrix secretion and cytoskeletal molecules expression in the neonatal offspring of pregnant mice with circadian disruption. An environmental circadian disruption model was constructed in 10- to 12-week-old pregnant mice. a The mandibles of the offspring (PN5) were dissected. Compared to the control group, the enamel matrix secretion decreased (shown as black arrows), and there were some obvious voids (shown as black asterisks) among ameloblasts of mandibular incisor germs in the disturbance group. Bar = 20 mum; sa secretory ameloblast, e enamel, d dentin, od odontoblast, dp dental pulp. b Compared to the control group, the expression levels of CK14 and F-actin were reduced in ameloblasts, especially in Tomes' processes in the disturbance group (shown as white arrows in the Con group and white asterisks in the disturbance group). Original magnification, x100

Supportive validation

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Experimental details: Figure 3 GATA4 depleted HS acquires stratified squamous epithelial cell gene expression. ( A ) qRT-PCR with epithelial cells from E18.5 control and G4 cKO mutant HS showed induced expression of genes associated with stratified squamous epithelium in mutants (n = 3 control, 3 mutant). Error bars show SEM. P values determined by a Student's t test, * P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 3 Figure Treatment of TRb-expressing MDA-MB-468 cells with T 3 represses cytokeratin genes. (A) Gene Set Enrichment Analysis performed between T 3 -treated 468-EV and 468-TRbeta expression analysis revealed Gene Ontology Biological Processes or Hallmark gene sets involving differentially expressed cytokeratins genes (adjusted p value < 1e-7). (B) Heatmap of all expressed KRT genes in 468-EV or 468-TRbeta cells following Vehicle or 10 nM T 3 treatment. (C) Normalized gene expression bar plots of the cytokeratin genes KRT5 and KRT14 from RNA-seq data, n = 3 for each condition. (D) and (E) Protein expression of KRT5 and KRT14 were evaluated by western blot, compared with beta-Actin as a loading control. The blot presented is representative of three independent experiments. **** p < 0.0001. TRbeta, thyroid hormone receptor beta [Color figure can be viewed at wileyonlinelibrary.com ]

Supportive validation

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Experimental details: FIGURE 2 Induced ameloblast lineages from Epfn-iPSCs following a three-stage induction protocol. (A) Diagram of the three-stage ameloblast induction protocol. (B) Stain of keratin 14 (KRT14), amelogenin (AMGN), and Alizarin Red S (ARS) on day 17 after ameloblast induction. Scale bars: 1 cm and 100 mum for upper and lower panels, respectively. (C,D) Stage-specific marker gene expression during the stepwise ameloblast induction of Epfn-iPSCs by RT-PCR (C) and real-time PCR (D) . Different letters among groups (e.g., A,B ) in Figure D indicate significant differences ( p < 0.05; one-way ANOVA and Tukey's test; n = 3). (E) Summary of gene expression in Figure C and D.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Deprivation of NO induces bi-lineage phenotype and expression of stem cell markers in mammary epithelial cells both in vivo and in 3D cultures. ( A ) CK14 (basal) and CK8/18 (luminal) staining of mouse mammary glands treated with control (PBS), L-arginine or L-NAME for 6 weeks. Co-localization of CK14 and CK8/18 was captured with ImageJ. Scale bars: 100 mum. (See the quantification in Supplementary Fig. 6A ). ( B ) Representative images of drug-treated mammary glands stained for stem cell markers: alkaline phosphatase (AP) (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 mum. (See the quantification in Supplementary Fig. 6B-D ). ( C ) CK14 and CK8/18 staining of non-malignant MCF10A cells cultivated in 3D ECM in the presence of control (PBS), L-arginine or L-NAME for 3 weeks. Scale bars: 50 mum. (See the quantification in Supplementary Fig. 6E ). ( D ) Drug-treated MCF10A cells in 3D cultures stained for AP (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 mum. (See the quantification in Supplementary Fig. 6F-H).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Deprivation of NO elevates self-renewal potential of non-malignant mammary epithelial cells. ( A ) Representative images of mammospheres derived from MCF10A cells grown on PolyHEMA-coated plates under the treatment of control (PBS), L-arginine or L-NAME for one week. Scale bars: 200 mum (top 2 rows) and 50 mum (bottom row). ( B ) The size of mammospheres derived from drug-treated MCF10A cells. ( C ) The number of spheroids formed at each seeding number. (See the micrographs of spheroids in Supplementary Fig. 7A ). ( D ) Mammosphere forming efficiency at each seeding number. Mammosphere forming efficiency (%) = (# of spheroids)/(# of cells seeded) x 100 119 , 120 . Statistical significance between different treatments was determined by two-way ANOVA analysis with Bonferroni post hoc test 175 . Error bars: mean +- STDEV. **p < 0.01; ***p < 0.001. ( E ) Staining of mammospheres for the basal marker, CK14, and luminal marker, CK8/18. Co-localization of CK14 and CK8/18 was captured with ImageJ. (See the quantification in Supplementary Fig. 7B ). ( F ) Limiting dilution analysis to determine the sphere forming frequency at clonal densities (>=200 cells/96-well) 126 . (Top) The graph plotted with the Y-axis as Ln (fraction of negative wells) and X-axis as seeding number. (Bottom) The sphere forming frequency corresponding to -(slope) of the line of best fit. ( G ) Staining of paraffin-embedded/sectioned mammospheres 129 for stem cell markers CD44 and CD24. Scale bars: 20

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3. Photomicrographs of P3 CD105 + K14 + cells after culture in adipogenic ( a ), osteogenic ( b ), and neurogenic ( c ) induction medium. Cells cultured in stromal medium for the same durations are also shown ( d - f ). Cells were stained oil red O ( a , d ), alizarin red ( b , e ), or anti-Map 2 and anti-mouse IgG-488 (green, c , f ). Nuclei were stained with DAPI (blue, c , f ). Bar = 50 µm ( a , b ); 100 mum ( c - f ).