Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX132160 - Provider product page
- Provider
- GeneTex
- Product name
- p130Cas (phospho Tyr165) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody (GTX132160) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.