675602

antibody from BioLegend

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

675602

Provider

BioLegend

Proper citation

BioLegend Cat#675602, RRID:AB_2565605

Product name

Purified anti-CDKN2A (p16)

Antibody type

Monoclonal

Antigen

Full length human CDKN2A recombinant protein expressed in E. coli.

Description

Cyclin-dependent kinase inhibitor 2A (CDKN2A) isoform 1, also known as p16-INK4a, is an inhibitor of the cyclin dependent kinases CDK4 and CDK6. CDKN2A acts as a negative regulator of cell cycle progression by preventing phosphorylation of the retinoblastoma protein (pRb) by cyclin-dependent kinases. CDKN2A expression is upregulated during cellular senescence and aging. It functions as a tumor suppressor to induce cell cycle arrest and aging of normal tissues. Germ line point mutations, homozygous deletion, or promoter methylation in the human CDKN2A locus causes uncontrolled cell proliferation, which results in a variety of malignancies such as melanoma, pancreatic adenocarcinoma, gastric cancer, and esophageal cancer.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

15C10C30

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from 293T cells (lane 1) and Jurkat cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-CDKN2A (clone 15C10C30) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.

Conjugate

Unconjugated