V3807

antibody from NSJ Bioreagents

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3807

Provider

NSJ Bioreagents

Product name

p14ARF Antibody / CDKN2A

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

4C6/4

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the p14ARF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain with p14ARF antibody (clone 4C6/4). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat brain with p14ARF antibody (clone 4C6/4). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free p14ARF antibody (clone 4C6/4) as confirmation of integrity and purity.