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antibody from Invitrogen Antibodies
Targeting: CDKN2A
ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA1-16639 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p14ARF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- p14ARF tends to run slightly higher than the theoretical MW of 14 kDa.
- Concentration
- 1 mg/mL
Submitted references Molecular Consequences of Depression Treatment: A Potential In Vitro Mechanism for Antidepressants-Induced Reprotoxic Side Effects.
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Sołek P, Mytych J, Tabęcka-Łonczyńska A, Koziorowski M
International journal of molecular sciences 2021 Nov 1;22(21)
International journal of molecular sciences 2021 Nov 1;22(21)
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Mytych J, Wos I, Solek P, Koziorowski M
Experimental cell research 2017 Jan 15;350(2):358-367
Experimental cell research 2017 Jan 15;350(2):358-367
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of p14ARF Antibody in HeLa whole cell lysate (lot C).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p14ARF in HeLa whole Cell lysate. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639). ECL detection method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) followed by DyLight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of p14ARF Antibody in HeLa cells. Panel one is a brightfield image, panel two shows the immunofluorescence staining of p14RF detected using Dylight 488 and panel three is an overlay of showing the nuclear localization of the p14ARF staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of p14ARF in HeLa cells. Samples were incubated in p14ARF polyclonal antibody (Product # PA1-16639) using a dilution of 1:400. Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Antidepressant-induced cell cycle profile regulation through related genes activation. GC-1 spg and GC-2 spd cells were treated with antidepressants for 48 and 96 h, the cell cycle profile ( A , B , D , E ) was determined and the level of p16, p21, p27, p53 proteins was controlled by Western blot technique. Representative blots are shown ( C , F ). Statistical differences were determined using one-way analysis of variance (ANOVA) with Dunnett's post-hoc test; p values < 0.05 were considered statistically significant. Asterisks (*) indicate the comparison between control and antidepressants-treated cells, whereas carets (^) indicate the comparison between the same drugs in different periods (48 vs. 96). Bars indicate mean +- SD, n = 3, ***/^^^ p < 0.001, **/^^ p < 0.01, */^ p < 0.05, no indication-no statistical significance.
