NB200-106

antibody from Novus Biologicals

Targeting: CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf

Provider product page for NB200-106

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [42]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: NB200-106 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB200-106, RRID:AB_10080908
Product name: Rabbit Polyclonal p16INK4a/CDKN2A Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified.
Reactivity: Mouse
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

CDKN2A protein structure - NB200-106 shown in red.

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Stem cells international 2019;2019:6257537

p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.

Novais EJ, Diekman BO, Shapiro IM, Risbud MV
Matrix biology : journal of the International Society for Matrix Biology 2019 Sep;82:54-70

COUP-TFII regulates satellite cell function and muscular dystrophy.

Xie X, Tsai SY, Tsai MJ
The Journal of clinical investigation 2016 Oct 3;126(10):3929-3941

E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3.

Chen D, Chen Y, Forrest D, Bremner R
Cell death and differentiation 2013 Jul;20(7):931-40

Epigenetic antagonism between polycomb and SWI/SNF complexes during oncogenic transformation.

Wilson BG, Wang X, Shen X, McKenna ES, Lemieux ME, Cho YJ, Koellhoffer EC, Pomeroy SL, Orkin SH, Roberts CW
Cancer cell 2010 Oct 19;18(4):316-28

Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis.

Wang WH, Hullinger RL, Andrisani OM
The Journal of biological chemistry 2008 Sep 12;283(37):25455-67

mTORC1 promotes survival through translational control of Mcl-1.

Mills JR, Hippo Y, Robert F, Chen SM, Malina A, Lin CJ, Trojahn U, Wendel HG, Charest A, Bronson RT, Kogan SC, Nadon R, Housman DE, Lowe SW, Pelletier J
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 5;105(31):10853-8

E2f1, E2f2, and E2f3 control E2F target expression and cellular proliferation via a p53-dependent negative feedback loop.

Timmers C, Sharma N, Opavsky R, Maiti B, Wu L, Wu J, Orringer D, Trikha P, Saavedra HI, Leone G
Molecular and cellular biology 2007 Jan;27(1):65-78

Early aging-associated phenotypes in Bub3/Rae1 haploinsufficient mice.

Baker DJ, Jeganathan KB, Malureanu L, Perez-Terzic C, Terzic A, van Deursen JM
The Journal of cell biology 2006 Feb 13;172(4):529-40

Increased DNA damage sensitivity and apoptosis in cells lacking the Snf5/Ini1 subunit of the SWI/SNF chromatin remodeling complex.

Klochendler-Yeivin A, Picarsky E, Yaniv M
Molecular and cellular biology 2006 Apr;26(7):2661-74

Regulation of the Arf/p53 tumor surveillance network by E2F.

Iaquinta PJ, Aslanian A, Lees JA
Cold Spring Harbor symposia on quantitative biology 2005;70:309-16

Human cytomegalovirus IE1-72 activates ataxia telangiectasia mutated kinase and a p53/p21-mediated growth arrest response.

Castillo JP, Frame FM, Rogoff HA, Pickering MT, Yurochko AD, Kowalik TF
Journal of virology 2005 Sep;79(17):11467-75

Immortalized fibroblasts from NF-kappaB RelA knockout mice show phenotypic heterogeneity and maintain increased sensitivity to tumor necrosis factor alpha after transformation by v-Ras.

Gapuzan ME, Schmah O, Pollock AD, Hoffmann A, Gilmore TD
Oncogene 2005 Sep 29;24(43):6574-83

p400 is required for E1A to promote apoptosis.

Samuelson AV, Narita M, Chan HM, Jin J, de Stanchina E, McCurrach ME, Narita M, Fuchs M, Livingston DM, Lowe SW
The Journal of biological chemistry 2005 Jun 10;280(23):21915-23

Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function.

Korgaonkar C, Hagen J, Tompkins V, Frazier AA, Allamargot C, Quelle FW, Quelle DE
Molecular and cellular biology 2005 Feb;25(4):1258-71

Resistance of CD1d-/- mice to ultraviolet-induced skin cancer is associated with increased apoptosis.

Matsumura Y, Moodycliffe AM, Nghiem DX, Ullrich SE, Ananthaswamy HN
The American journal of pathology 2004 Sep;165(3):879-87

p19ARF determines the balance between normal cell proliferation rate and apoptosis during mammary gland development.

Yi Y, Shepard A, Kittrell F, Mulac-Jericevic B, Medina D, Said TK
Molecular biology of the cell 2004 May;15(5):2302-11

Growth retardation and premature aging phenotypes in mice with disruption of the SNF2-like gene, PASG.

Sun LQ, Lee DW, Zhang Q, Xiao W, Raabe EH, Meeker A, Miao D, Huso DL, Arceci RJ
Genes & development 2004 May 1;18(9):1035-46

Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics.

Aslanian A, Iaquinta PJ, Verona R, Lees JA
Genes & development 2004 Jun 15;18(12):1413-22

BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice.

Baker DJ, Jeganathan KB, Cameron JD, Thompson M, Juneja S, Kopecka A, Kumar R, Jenkins RB, de Groen PC, Roche P, van Deursen JM
Nature genetics 2004 Jul;36(7):744-9

The differential impact of p16(INK4a) or p19(ARF) deficiency on cell growth and tumorigenesis.

Sharpless NE, Ramsey MR, Balasubramanian P, Castrillon DH, DePinho RA
Oncogene 2004 Jan 15;23(2):379-85

Active Akt and functional p53 modulate apoptosis in Abelson virus-transformed pre-B cells.

Gong L, Unnikrishnan I, Raghavan A, Parmar K, Rosenberg N
Journal of virology 2004 Feb;78(4):1636-44

Sensitization to the lysosomal cell death pathway upon immortalization and transformation.

Fehrenbacher N, Gyrd-Hansen M, Poulsen B, Felbor U, Kallunki T, Boes M, Weber E, Leist M, Jäättelä M
Cancer research 2004 Aug 1;64(15):5301-10

p16(Ink4a) interferes with Abelson virus transformation by enhancing apoptosis.

Sachs Z, Sharpless NE, DePinho RA, Rosenberg N
Journal of virology 2004 Apr;78(7):3304-11

Macrophage migration inhibitory factor deficiency is associated with altered cell growth and reduced susceptibility to Ras-mediated transformation.

Petrenko O, Fingerle-Rowson G, Peng T, Mitchell RA, Metz CN
The Journal of biological chemistry 2003 Mar 28;278(13):11078-85

Absence of p53 complements defects in Abelson murine leukemia virus signaling.

Unnikrishnan I, Rosenberg N
Journal of virology 2003 Jun;77(11):6208-15

Direct coupling of the cell cycle and cell death machinery by E2F.

Nahle Z, Polakoff J, Davuluri RV, McCurrach ME, Jacobson MD, Narita M, Zhang MQ, Lazebnik Y, Bar-Sagi D, Lowe SW
Nature cell biology 2002 Nov;4(11):859-64

Insulin-like growth factor I induces MDM2-dependent degradation of p53 via the p38 MAPK pathway in response to DNA damage.

Héron-Milhavet L, LeRoith D
The Journal of biological chemistry 2002 May 3;277(18):15600-6

A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy.

Schmitt CA, Fridman JS, Yang M, Lee S, Baranov E, Hoffman RM, Lowe SW
Cell 2002 May 3;109(3):335-46

Hepatitis B virus X protein differentially regulates cell cycle progression in X-transforming versus nontransforming hepatocyte (AML12) cell lines.

Lee S, Tarn C, Wang WH, Chen S, Hullinger RL, Andrisani OM
The Journal of biological chemistry 2002 Mar 8;277(10):8730-40

Role of the alternating reading frame (P19)-p53 pathway in an in vivo murine colon tumor model.

Nambiar PR, Giardina C, Guda K, Aizu W, Raja R, Rosenberg DW
Cancer research 2002 Jul 1;62(13):3667-74

Germ line transmission of the Cdk4(R24C) mutation facilitates tumorigenesis and escape from cellular senescence.

Rane SG, Cosenza SC, Mettus RV, Reddy EP
Molecular and cellular biology 2002 Jan;22(2):644-56

Obligate roles for p16(Ink4a) and p19(Arf)-p53 in the suppression of murine pancreatic neoplasia.

Bardeesy N, Morgan J, Sinha M, Signoretti S, Srivastava S, Loda M, Merlino G, DePinho RA
Molecular and cellular biology 2002 Jan;22(2):635-43

Early gene expression changes preceding thyroid hormone-induced involution of a thyrotrope tumor.

Wood WM, Sarapura VD, Dowding JM, Woodmansee WW, Haakinson DJ, Gordon DF, Ridgway EC
Endocrinology 2002 Feb;143(2):347-59

Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis.

Sharpless NE, Bardeesy N, Lee KH, Carrasco D, Castrillon DH, Aguirre AJ, Wu EA, Horner JW, DePinho RA
Nature 2001 Sep 6;413(6851):86-91

Inactivation of both Rb and p53 pathways in mouse lung epithelial cell lines.

McDoniels-Silvers AL, Herzog CR, Tyson FL, Malkinson AM, You M
Experimental lung research 2001 Apr-May;27(3):297-318

Oncogenic ras activates the ARF-p53 pathway to suppress epithelial cell transformation.

Lin AW, Lowe SW
Proceedings of the National Academy of Sciences of the United States of America 2001 Apr 24;98(9):5025-30

The absence of Msh2 alters abelson virus pre-B-cell transformation by influencing p53 mutation.

Jenab-Wolcott J, Rodriguez-Correa D, Reitmair AH, Mak T, Rosenberg N
Molecular and cellular biology 2000 Nov;20(22):8373-81

Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway.

Tournier C, Hess P, Yang DD, Xu J, Turner TK, Nimnual A, Bar-Sagi D, Jones SN, Flavell RA, Davis RJ
Science (New York, N.Y.) 2000 May 5;288(5467):870-4

Differential requirement for p19ARF in the p53-dependent arrest induced by DNA damage, microtubule disruption, and ribonucleotide depletion.

Khan SH, Moritsugu J, Wahl GM
Proceedings of the National Academy of Sciences of the United States of America 2000 Mar 28;97(7):3266-71

Targeted disruption of the three Rb-related genes leads to loss of G(1) control and immortalization.

Sage J, Mulligan GJ, Attardi LD, Miller A, Chen S, Williams B, Theodorou E, Jacks T
Genes & development 2000 Dec 1;14(23):3037-50

Dysphagia as a major symptom of tetanus.

Ross J, Murrant NJ
The Journal of laryngology and otology 1992 Oct;106(10):923-4

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of FFPE mouse spleen using p16INK4a/CDKN2A antibody at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.