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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000924-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000924-M04, RRID:AB_626404
- Product name
- CD7 monoclonal antibody (M04), clone 1B8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CD7.
- Antigen sequence
PGALAAQEVQQSPHCTTVPVGASVNITCSTSGGLR
GIYLRQLGPQPQDIIYYEDGVVPTTDRRFRGRIDF
SGSQDNLTITMHRLQLSDTGTYTCQAITEVNVYGS
GTLVLVTEEQSQGWHRCSDAPPRASALPAPPTGSA
LPDPQTASALPDPPAASALPAALAVISFLLGLGLG
VACVLARTQIKKLCSWRDKNSAACVVYEDMSHSRC
NTLSSPNQYQ- Isotype
- IgG
- Antibody clone number
- 1B8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CD7 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD7 transfected lysate using anti-CD7 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CD7 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
