A03260-3

antibody from Boster Biological Technology

Targeting: CLDN5 AWAL, BEC1, CPETRL1, TMVCF

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A03260-3

Provider

Boster Biological Technology

Product name

Anti-Claudin 5/CLDN5 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Claudin 5/CLDN5 detection. Tested with IHC-P, IF in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Brain Infection by Hepatitis E Virus Probably via Damage of the Blood-Brain Barrier Due to Alterations of Tight Junction Proteins.

Tian J, Shi R, Liu T, She R, Wu Q, An J, Hao W, Soomro MH
Frontiers in cellular and infection microbiology 2019;9:52

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Claudin 5/CLDN5 using anti-Claudin 5/CLDN5 antibody (A03260-3). Claudin 5/CLDN5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 5/CLDN5 Antibody (A03260-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Additional image