329494

antibody from Invitrogen Antibodies

Targeting: CLDN4 CPE-R, CPETR, CPETR1, hCPE-R, WBSCR8

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

329494

Provider

Invitrogen Antibodies

Product name

Claudin 4 Monoclonal Antibody (3E2C1), Alexa Fluor™ 594

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

3E2C1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells.

Cong X, Zhang Y, Li J, Mei M, Ding C, Xiang RL, Zhang LW, Wang Y, Wu LL, Yu GY
Journal of cell science 2015 Jun 15;128(12):2271-86

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Universal antibody conjugation to nanoparticles using the Fcγ receptor I (FcγRI): quantitative profiling of membrane biomarkers.

Kim C, Galloway JF, Lee KH, Searson PC
Bioconjugate chemistry 2014 Oct 15;25(10):1893-901

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CLAUDIN4AF594 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with CLAUDIN4AF594 Mouse Monoclonal Antibody (Product # 32-9494) at 1 µg - 2 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) panel c. Panel d is a merged image showing cytoplasmic localization of CLAUDIN4AF594. Panel e shows no primary antibody. The images were captured at 20X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Quantitative cell targeting using QD-FcR-Ab conjugates. (A) phaseand fluorescence images of Panc-1 cells incubated with different concentrationsof QD-FcR-aCLDN4 conjugates. (B) The fluorescence of known concentrationsof QD suspensions is used for calibration (see Materialsand Methods for details). (C) Calibration curve showing averagefluorescence intensity per unit area versus QD concentration per unitarea. The calibration curve is used to convert the fluorescence intensityper unit area on the cell surface to an expression level per unitarea. (D) Average fluorescence intensity per unit area for CLDN4 expressionon Panc-1 cells versus QD concentration. Error bars represent standarderror. Each point represents the average intensity from at least 200cells.