PA1001

antibody from Boster Biological Technology

Targeting: ABI1 ABI-1, E3B1, SSH3BP1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1001

Provider

Boster Biological Technology

Product name

Anti-SSH3BP1/ABI1 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for SSH3BP1/ABI1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IF. Reactive species: Human. SSH3BP1/ABI1 information: Molecular Weight: 55081 MW; Subcellular Localization: Cytoplasm . Nucleus . Cell projection, lamellipodium . Cell projection, filopodium . Cell projection, growth cone . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cytoplasm, cytoskeleton . Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity); Tissue Specificity: Widely expressed, with highest expression in brain.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IF analysis of ABI using anti- ABI antibody (PA1001) ABI was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- ABI Antibody (PA1001) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Additional image