Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-59845 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59845, RRID:AB_892454
- Product name
- Rabbit Polyclonal ABI1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Long-Term Exposure to Imatinib Mesylate Downregulates Hippo Pathway and Activates YAP in a Model of Chronic Myelogenous Leukemia.
Chorzalska A, Kim JF, Roder K, Tepper A, Ahsan N, Rao RSP, Olszewski AJ, Yu X, Terentyev D, Morgan J, Treaba DO, Zhao TC, Liang O, Gruppuso PA, Dubielecka PM
Stem cells and development 2017 May 1;26(9):656-677
Stem cells and development 2017 May 1;26(9):656-677
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABI1 Antibody [NB100-59845] - Detection of Human ABI1 on HeLa whole cell lysate using NB100-59845. ABI1 was also immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABI1 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB