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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX113561 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113561, RRID:AB_10620222
- Product name
- Transgelin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
Submitted references MRTF-A signaling regulates the acquisition of the contractile phenotype in dedifferentiated chondrocytes.
Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.
Laminin regulates PDGFRβ(+) cell stemness and muscle development.
Integrated analysis of mRNA, microRNA and protein in systemic lupus erythematosus-specific induced pluripotent stem cells from urine.
Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
Proteomic analysis identifies cytoskeleton-interacting proteins as major downstream targets of altered folate status in the aorta of adult rat.
Parreno J, Raju S, Wu PH, Kandel RA
Matrix biology : journal of the International Society for Matrix Biology 2017 Oct;62:3-14
Matrix biology : journal of the International Society for Matrix Biology 2017 Oct;62:3-14
Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.
Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV, Wanner IB
Glia 2016 May;64(5):668-94
Glia 2016 May;64(5):668-94
Laminin regulates PDGFRβ(+) cell stemness and muscle development.
Yao Y, Norris EH, Mason CE, Strickland S
Nature communications 2016 May 3;7:11415
Nature communications 2016 May 3;7:11415
Integrated analysis of mRNA, microRNA and protein in systemic lupus erythematosus-specific induced pluripotent stem cells from urine.
Tang D, Chen Y, He H, Huang J, Chen W, Peng W, Lu Q, Dai Y
BMC genomics 2016 Jul 11;17:488
BMC genomics 2016 Jul 11;17:488
Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
Yao Y, Chen ZL, Norris EH, Strickland S
Nature communications 2014 Mar 3;5:3413
Nature communications 2014 Mar 3;5:3413
Proteomic analysis identifies cytoskeleton-interacting proteins as major downstream targets of altered folate status in the aorta of adult rat.
Gérard N, Chanson-Rollé A, Rock E, Brachet P
Molecular nutrition & food research 2014 Dec;58(12):2307-19
Molecular nutrition & food research 2014 Dec;58(12):2307-19
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Transgelin (GTX113561, upper panel) and beta-actin (lower panel)? Sample (35 ?g of whole cell lysate)? A: A-10 cell B: A-10 cell treated with MG132 GTX113561 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse liver 12% SDS PAGE GTX113561 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 12% SDS PAGE Transgelin antibody GTX113561 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transgelin antibody detects Transgelin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted by 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transgelin antibody detects Transgelin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Transgelin protein stained by Transgelin antibody (GTX113561) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transgelin antibody detects Transgelin protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Transgelin antibody (GTX113561) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Transgelin antibody detects Transgelin protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Transgelin antibody (GTX113561) dilution: 1:500.
