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LearnNB600-507
antibody from Novus Biologicals
Targeting: TAGLN
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB600-507 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-507, RRID:AB_10002969
- Product name
- Goat Polyclonal Transgelin/TAGLN/SM22 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NP_003177.2 and NP_001001522.1 represent identical protein.The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumor suppressor.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis.
Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.
Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells.
Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat.
Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation.
Vascular Smooth Muscle-Specific EP4 Receptor Deletion in Mice Exacerbates Angiotensin II-Induced Renal Injury.
Mechanical activation of angiotensin II type 1 receptors causes actin remodelling and myogenic responsiveness in skeletal muscle arterioles.
The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells.
α5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries.
PKC-mediated cerebral vasoconstriction: Role of myosin light chain phosphorylation versus actin cytoskeleton reorganization.
Nitric oxide and carbon monoxide antagonize TGF-β through ligand-independent internalization of TβR1/ALK5.
Cytoskeletal reorganization evoked by Rho-associated kinase- and protein kinase C-catalyzed phosphorylation of cofilin and heat shock protein 27, respectively, contributes to myogenic constriction of rat cerebral arteries.
Transforming growth factor-β regulates endothelial function during high salt intake in rats.
Ca2+ sensitization due to myosin light chain phosphatase inhibition and cytoskeletal reorganization in the myogenic response of skeletal muscle resistance arteries.
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation.
High-phosphate-induced calcification is related to SM22α promoter methylation in vascular smooth muscle cells.
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.
Myosin, transgelin, and myosin light chain kinase: expression and function in asthma.
Proteomic profiling of chronic low-frequency stimulated fast muscle.
Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle.
Takagaki Y, Lee SM, Dongqing Z, Kitada M, Kanasaki K, Koya D
Autophagy 2020 Oct;16(10):1905-1914
Autophagy 2020 Oct;16(10):1905-1914
Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.
Li J, Liu H, Takagi S, Nitta K, Kitada M, Srivastava SP, Takagaki Y, Kanasaki K, Koya D
JCI insight 2020 Mar 26;5(6)
JCI insight 2020 Mar 26;5(6)
Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells.
Deng JT, Bhaidani S, Sutherland C, MacDonald JA, Walsh MP
PloS one 2019;14(12):e0226406
PloS one 2019;14(12):e0226406
Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat.
Abd-Elrahman KS, Colinas O, Walsh EJ, Zhu HL, Campbell CM, Walsh MP, Cole WC
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Jan;37(1):227-240
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Jan;37(1):227-240
Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation.
Herencia C, Diaz-Tocados JM, Jurado L, Montes de Oca A, Rodríguez-Ortiz ME, Martín-Alonso C, Martínez-Moreno JM, Vergara N, Rodríguez M, Almadén Y, Muñoz-Castañeda JR
PloS one 2016;11(6):e0156788
PloS one 2016;11(6):e0156788
Vascular Smooth Muscle-Specific EP4 Receptor Deletion in Mice Exacerbates Angiotensin II-Induced Renal Injury.
Thibodeau JF, Holterman CE, He Y, Carter A, Cron GO, Boisvert NC, Abd-Elrahman KS, Hsu KJ, Ferguson SS, Kennedy CR
Antioxidants & redox signaling 2016 Oct 20;25(12):642-656
Antioxidants & redox signaling 2016 Oct 20;25(12):642-656
Mechanical activation of angiotensin II type 1 receptors causes actin remodelling and myogenic responsiveness in skeletal muscle arterioles.
Hong K, Zhao G, Hong Z, Sun Z, Yang Y, Clifford PS, Davis MJ, Meininger GA, Hill MA
The Journal of physiology 2016 Dec 1;594(23):7027-7047
The Journal of physiology 2016 Dec 1;594(23):7027-7047
The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells.
Deng JT, Wang XL, Chen YX, O'Brien ER, Gui Y, Walsh MP
PloS one 2015;10(2):e0116969
PloS one 2015;10(2):e0116969
α5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries.
Colinas O, Moreno-Domínguez A, Zhu HL, Walsh EJ, Pérez-García MT, Walsh MP, Cole WC
Biochemical pharmacology 2015 Oct 1;97(3):281-91
Biochemical pharmacology 2015 Oct 1;97(3):281-91
PKC-mediated cerebral vasoconstriction: Role of myosin light chain phosphorylation versus actin cytoskeleton reorganization.
El-Yazbi AF, Abd-Elrahman KS, Moreno-Dominguez A
Biochemical pharmacology 2015 Jun 15;95(4):263-78
Biochemical pharmacology 2015 Jun 15;95(4):263-78
Nitric oxide and carbon monoxide antagonize TGF-β through ligand-independent internalization of TβR1/ALK5.
Hovater MB, Ying WZ, Agarwal A, Sanders PW
American journal of physiology. Renal physiology 2014 Sep 15;307(6):F727-35
American journal of physiology. Renal physiology 2014 Sep 15;307(6):F727-35
Cytoskeletal reorganization evoked by Rho-associated kinase- and protein kinase C-catalyzed phosphorylation of cofilin and heat shock protein 27, respectively, contributes to myogenic constriction of rat cerebral arteries.
Moreno-Domínguez A, El-Yazbi AF, Zhu HL, Colinas O, Zhong XZ, Walsh EJ, Cole DM, Kargacin GJ, Walsh MP, Cole WC
The Journal of biological chemistry 2014 Jul 25;289(30):20939-52
The Journal of biological chemistry 2014 Jul 25;289(30):20939-52
Transforming growth factor-β regulates endothelial function during high salt intake in rats.
Ying WZ, Aaron KJ, Sanders PW
Hypertension (Dallas, Tex. : 1979) 2013 Nov;62(5):951-6
Hypertension (Dallas, Tex. : 1979) 2013 Nov;62(5):951-6
Ca2+ sensitization due to myosin light chain phosphatase inhibition and cytoskeletal reorganization in the myogenic response of skeletal muscle resistance arteries.
Moreno-Domínguez A, Colinas O, El-Yazbi A, Walsh EJ, Hill MA, Walsh MP, Cole WC
The Journal of physiology 2013 Mar 1;591(5):1235-50
The Journal of physiology 2013 Mar 1;591(5):1235-50
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
Donati C, Marseglia G, Magi A, Serratì S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P
PloS one 2011;6(5):e20389
PloS one 2011;6(5):e20389
Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation.
Walsh MP, Thornbury K, Cole WC, Sergeant G, Hollywood M, McHale N
American journal of physiology. Renal physiology 2011 Jan;300(1):F73-85
American journal of physiology. Renal physiology 2011 Jan;300(1):F73-85
High-phosphate-induced calcification is related to SM22α promoter methylation in vascular smooth muscle cells.
Montes de Oca A, Madueño JA, Martinez-Moreno JM, Guerrero F, Muñoz-Castañeda J, Rodriguez-Ortiz ME, Mendoza FJ, Almaden Y, Lopez I, Rodriguez M, Aguilera-Tejero E
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2010 Sep;25(9):1996-2005
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2010 Sep;25(9):1996-2005
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.
Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P
Molecular biology of the cell 2010 Mar 15;21(6):1111-24
Molecular biology of the cell 2010 Mar 15;21(6):1111-24
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.
Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P
Cellular and molecular life sciences : CMLS 2009 May;66(10):1741-54
Cellular and molecular life sciences : CMLS 2009 May;66(10):1741-54
Myosin, transgelin, and myosin light chain kinase: expression and function in asthma.
Léguillette R, Laviolette M, Bergeron C, Zitouni N, Kogut P, Solway J, Kachmar L, Hamid Q, Lauzon AM
American journal of respiratory and critical care medicine 2009 Feb 1;179(3):194-204
American journal of respiratory and critical care medicine 2009 Feb 1;179(3):194-204
Proteomic profiling of chronic low-frequency stimulated fast muscle.
Donoghue P, Doran P, Wynne K, Pedersen K, Dunn MJ, Ohlendieck K
Proteomics 2007 Sep;7(18):3417-30
Proteomics 2007 Sep;7(18):3417-30
Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle.
Nishida W, Kitami Y, Abe M, Hiwada K
Biochemistry international 1991 Mar;23(4):663-8
Biochemistry international 1991 Mar;23(4):663-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of human placenta lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NB600-507] - Staining of Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
