NBP2-70454

antibody from Novus Biologicals

Targeting: CRYAB CRYA2, HSPB5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-70454

Provider

Novus Biologicals

Product name

Mouse Monoclonal AlphaB Crystallin/CRYAB Antibody

Antibody type

Monoclonal

Description

Immunogen affinity purified.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP2-70454)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454), and then analyzed by flow cytometry.