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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- R1565 - Provider product page
- Provider
- OriGene
- Product name
- Osteopontin (SPP1) rabbit polyclonal antibody, Serum
- Antibody type
- Polyclonal
- Description
- Osteopontin (SPP1) rabbit polyclonal antibody, Serum
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPP1
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- 75 mg/ml (by Refractometry)
Submitted references Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells.
Robertson BW, Chellaiah MA
Experimental cell research 2010 Jan 1;316(1):1-11
Experimental cell research 2010 Jan 1;316(1):1-11
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoblotting: Osteopontin antibody was used at a 1/1,000 dilution to detect Osteopontin by Western blot. In lane 2 reactivity is shown against 250 ng of Human osteopontin. Lane 3 shows reactivity of MMP-cleaved osteopontin. Lane 1 shows the position of molecular weight markers. Use a 1/10,000 dilution of HRP conjugated Goat anti-Rabbit IgG (R1454HRP) for detection.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Osteopontin antibody was used at a 1/100-1/300 dilution to detect Osteopontin which is a normal component of elastic fibers in the breast (shown heavily stained in this section of Human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Osteopontin cleavage diagram: OPN is cleaved by MMP to yield 2 fragments, which migrate at 40 kDa (N terminal) and 32 kDa (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs. The epitope that is recognized by Rabbit-anti-Osteopontin is shown in violet. This antibody detects the full length OPN and the 32 kDa fragment. It does not recognize the 40 kDa fragment.
- Validation comment
- Assay
