H00006696-D01P

antibody from Abnova Corporation

Targeting: SPP1 BNSP, BSPI, ETA-1, OPN

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006696-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006696-D01P, RRID:AB_1580111

Product name

SPP1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human SPP1 protein.

Antigen sequence

MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNK
YPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFK
QETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDS
NDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDL
PATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFR
RPDIQYPDATDEDITSHMESEELNGAYKAIPVAQD
LNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRL
YKRKANDESNEHSDVIDSQELSKVSREFHSHEFHS
HEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SPP1 expression in transfected 293T cell line (H00006696-T01) by SPP1 MaxPab polyclonal antibody.Lane 1: SPP1 transfected lysate(35.40 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between SPP1 and F2. HeLa cells were stained with anti-SPP1 rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)