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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10297 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10297, RRID:AB_1710539
- Product name
- SPP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SPP1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The immunology of Eta-1/osteopontin.
Osteopontin-induced modifications of cellular functions.
Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells.
Weber GF, Cantor H
Cytokine & growth factor reviews 1996 Oct;7(3):241-8
Cytokine & growth factor reviews 1996 Oct;7(3):241-8
Osteopontin-induced modifications of cellular functions.
Denhardt DT, Lopez CA, Rollo EE, Hwang SM, An XR, Walther SE
Annals of the New York Academy of Sciences 1995 Apr 21;760:127-42
Annals of the New York Academy of Sciences 1995 Apr 21;760:127-42
Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells.
Denhardt DT, Chambers AF
Journal of cellular biochemistry 1994 Sep;56(1):48-51
Journal of cellular biochemistry 1994 Sep;56(1):48-51
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1:1,000 dilution to detect SPP1 by Western blot.In lane 1, reactivity is shown against 250 ng of human SPP1.Lane 2 shows reactivity of MMP-cleaved SPP1.Use a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1 : 100-1 : 300 dilution to detect SPP1 by immunohistochemistry.SPP1 is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells).There is also weak staining of the extracellular matrix.SPP1 is not expressed in breast tumor cells, and there is no staining of the breast cells in this section.No antigen retrieval is required.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
