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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 14-9096-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Osteopontin Monoclonal Antibody (2F10), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 2F10 monoclonal antibody recognizes human osteopontin (OPN), a secreted glycophosphoprotein expressed by multiple cell types, including leukocytes, fibroblasts, dendritic cells, macrophages, myoblasts, bone-forming cells, and epithelial cells. OPN is an extracellular matrix protein with multiple roles, including bone resorption and calcification, immune system regulation, wound healing, and tumorigenesis. Thrombin cleavage of OPN allows binding of integrin receptors, expressed by immune cells, and subsequent modulation of cell adhesion, migration, and survival. OPN is expressed in bones, teeth, placenta, blood vessels, kidney, and various tumors. Osteopontin has been identified as a potential therapeutic target in the treatment of autoimmune disease, cancer metastasis, bone mineralization diseases, and osteoporosis.
- Antibody clone number
- 2F10
- Concentration
- 0.5 mg/mL
Submitted references Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
Association of osteopontin and cyclooxygenase-2 expression with breast cancer subtypes and their use as potential biomarkers.
Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer.
Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes DC, Panoutsakopoulou V
Proceedings of the National Academy of Sciences of the United States of America 2014 Mar 4;111(9):E856-65
Proceedings of the National Academy of Sciences of the United States of America 2014 Mar 4;111(9):E856-65
Association of osteopontin and cyclooxygenase-2 expression with breast cancer subtypes and their use as potential biomarkers.
Thorat D, Sahu A, Behera R, Lohite K, Deshmukh S, Mane A, Karnik S, Doke S, Kundu GC
Oncology letters 2013 Dec;6(6):1559-1564
Oncology letters 2013 Dec;6(6):1559-1564
Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer.
Plumer A, Duan H, Subramaniam S, Lucas FL, Miesfeldt S, Ng AK, Liaw L
BMC cancer 2008 Jan 31;8:38
BMC cancer 2008 Jan 31;8:38
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Osteopontin Monoclonal Antibody (2F10), eBioscience™(Product # 14-9096-82) and a 75kDa band corresponding to Osteopontin was observed in Hep G2 and MCF-7 cell line treated with Protein Transport Inhibitor (PTI, 1X for 4 hr) as compared to IMR-32 and Jurkat cell line treated with same inhibitor. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 +PTI (1X for 4 hr) (Lane 2), MCF-7 (Lane 3), MCF-7+PTI (1X for 4 hr) (Lane 4), IMR-32 ( Lane 5), IMR-32+ PTI (1X for 4 hr) (Lane 6), Jurkat (Lane 7) and Jurkat+ PTI (1X for 4 hr) ( Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of methanol-fixed MCF7 cells using 10 µg/mL of Anti-Human Osteopontin Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductile carcinoma tissue using 5 µg/mL of Anti-Human Osteopontin Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
