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- Antibody Data
- Antigen structure
- References [37]
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- Immunohistochemistry [1]
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- Product number
- MA5-11800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Involucrin Monoclonal Antibody (SY5), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-11800 targets Involucrin in ELISA, IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, Non-human primate, Porcine, and Rat samples. The MA5-11800 immunogen is human keratinocytes' involucrin.
- Reactivity
- Human, Rat, Canine, Porcine
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- SY5
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Growth and differentiation of HaCaT keratinocytes.
Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents.
Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes.
Saurian papulosis: a new clinicopathological entity.
Morphologic changes in photodamaged organotypic human skin culture after treatment of autologous adipose-derived stromal cells.
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
Low-temperature electrospun silk scaffold for in vitro mucosal modeling.
Id-1 induces cell invasiveness in immortalized epithelial cells by regulating cadherin switching and Rho GTPases.
CD44 regulates tight-junction assembly and barrier function.
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells.
Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model.
Differential gene expression in primary human skin keratinocytes and fibroblasts in response to ionizing radiation.
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients.
p63 Is essential for the proliferative potential of stem cells in stratified epithelia.
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes.
Gene transfer in human skin with different pseudotyped HIV-based vectors.
Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors.
Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation.
Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells.
Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite.
Long-term maintenance of human keratinocytes in vitro.
Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness.
Characterization of putative stem cell phenotype in human limbal epithelia.
Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis.
Enhanced redundancy in Akt and mitogen-activated protein kinase-induced survival of malignant versus normal prostate epithelial cells.
Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency.
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.
Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels.
Microtubule disruption in keratinocytes induces cell-cell adhesion through activation of endogenous E-cadherin.
Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2.
Wilson VG
Methods in molecular biology (Clifton, N.J.) 2014;1195:33-41
Methods in molecular biology (Clifton, N.J.) 2014;1195:33-41
Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents.
Mainzer C, Barrichello C, Debret R, Remoué N, Sigaudo-Roussel D, Sommer P
International journal of cosmetic science 2014 Oct;36(5):427-35
International journal of cosmetic science 2014 Oct;36(5):427-35
Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes.
Pontiggia L, Klar A, Böttcher-Haberzeth S, Biedermann T, Meuli M, Reichmann E
Pediatric surgery international 2013 Mar;29(3):249-56
Pediatric surgery international 2013 Mar;29(3):249-56
Saurian papulosis: a new clinicopathological entity.
Molina-Ruiz AM, del Carmen Fariña M, Carrasco L, Santonja C, Rodríguez-Peralto JL, Torrelo A, Kutzner H, Requena L
Journal of the American Academy of Dermatology 2013 Feb;68(2):e17-22
Journal of the American Academy of Dermatology 2013 Feb;68(2):e17-22
Morphologic changes in photodamaged organotypic human skin culture after treatment of autologous adipose-derived stromal cells.
Dhong ES, Hwang NH, Kim DW, Rajashekhar G, Johnstone BH, March KL
The Journal of craniofacial surgery 2012 May;23(3):805-11
The Journal of craniofacial surgery 2012 May;23(3):805-11
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
Mitchell D, Israr M, Alam S, Kishel J, Dinello D, Meyers C
HIV medicine 2012 May;13(5):276-90
HIV medicine 2012 May;13(5):276-90
Low-temperature electrospun silk scaffold for in vitro mucosal modeling.
Bulysheva AA, Bowlin GL, Klingelhutz AJ, Yeudall WA
Journal of biomedical materials research. Part A 2012 Mar;100(3):757-67
Journal of biomedical materials research. Part A 2012 Mar;100(3):757-67
Id-1 induces cell invasiveness in immortalized epithelial cells by regulating cadherin switching and Rho GTPases.
Cheung PY, Yip YL, Tsao SW, Ching YP, Cheung AL
Journal of cellular biochemistry 2011 Jan;112(1):157-68
Journal of cellular biochemistry 2011 Jan;112(1):157-68
CD44 regulates tight-junction assembly and barrier function.
Kirschner N, Haftek M, Niessen CM, Behne MJ, Furuse M, Moll I, Brandner JM
The Journal of investigative dermatology 2011 Apr;131(4):932-43
The Journal of investigative dermatology 2011 Apr;131(4):932-43
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
Qi H, Zheng X, Yuan X, Pflugfelder SC, Li DQ
Journal of cellular physiology 2010 Oct;225(1):180-5
Journal of cellular physiology 2010 Oct;225(1):180-5
Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells.
Tharakan S, Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Schiestl C, Reichmann E, Meuli M
Pediatric surgery international 2010 Jan;26(1):71-7
Pediatric surgery international 2010 Jan;26(1):71-7
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
Metallo CM, Azarin SM, Moses LE, Ji L, de Pablo JJ, Palecek SP
Tissue engineering. Part A 2010 Jan;16(1):213-23
Tissue engineering. Part A 2010 Jan;16(1):213-23
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M, Reichmann E
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells.
Bonfanti P, Claudinot S, Amici AW, Farley A, Blackburn CC, Barrandon Y
Nature 2010 Aug 19;466(7309):978-82
Nature 2010 Aug 19;466(7309):978-82
Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model.
Beckstead BL, Tung JC, Liang KJ, Tavakkol Z, Usui ML, Olerud JE, Giachelli CM
Journal of biomedical materials research. Part A 2009 Nov;91(2):436-46
Journal of biomedical materials research. Part A 2009 Nov;91(2):436-46
Differential gene expression in primary human skin keratinocytes and fibroblasts in response to ionizing radiation.
Warters RL, Packard AT, Kramer GF, Gaffney DK, Moos PJ
Radiation research 2009 Jul;172(1):82-95
Radiation research 2009 Jul;172(1):82-95
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
Gröger S, Michel J, Meyle J
Journal of periodontal research 2008 Dec;43(6):604-14
Journal of periodontal research 2008 Dec;43(6):604-14
Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients.
Araya J, Cambier S, Markovics JA, Wolters P, Jablons D, Hill A, Finkbeiner W, Jones K, Broaddus VC, Sheppard D, Barzcak A, Xiao Y, Erle DJ, Nishimura SL
The Journal of clinical investigation 2007 Nov;117(11):3551-62
The Journal of clinical investigation 2007 Nov;117(11):3551-62
p63 Is essential for the proliferative potential of stem cells in stratified epithelia.
Senoo M, Pinto F, Crum CP, McKeon F
Cell 2007 May 4;129(3):523-36
Cell 2007 May 4;129(3):523-36
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
Islam MS, Zhou H
European journal of dermatology : EJD 2007 Jul-Aug;17(4):302-8
European journal of dermatology : EJD 2007 Jul-Aug;17(4):302-8
Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes.
Hayashi N, Kido J, Kido R, Wada C, Kataoka M, Shinohara Y, Nagata T
Journal of periodontal research 2007 Feb;42(1):1-7
Journal of periodontal research 2007 Feb;42(1):1-7
Gene transfer in human skin with different pseudotyped HIV-based vectors.
Hachiya A, Sriwiriyanont P, Patel A, Saito N, Ohuchi A, Kitahara T, Takema Y, Tsuboi R, Boissy RE, Visscher MO, Wilson JM, Kobinger GP
Gene therapy 2007 Apr;14(8):648-56
Gene therapy 2007 Apr;14(8):648-56
Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors.
Chen Z, Mok H, Pflugfelder SC, Li DQ, Barry MA
Experimental eye research 2006 Oct;83(4):798-806
Experimental eye research 2006 Oct;83(4):798-806
Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation.
Beckstead BL, Santosa DM, Giachelli CM
Journal of biomedical materials research. Part A 2006 Oct;79(1):94-103
Journal of biomedical materials research. Part A 2006 Oct;79(1):94-103
Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells.
Chen Z, Evans WH, Pflugfelder SC, Li DQ
Stem cells (Dayton, Ohio) 2006 May;24(5):1265-73
Stem cells (Dayton, Ohio) 2006 May;24(5):1265-73
Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite.
Sanmano B, Mizoguchi M, Suga Y, Ikeda S, Ogawa H
Journal of dermatological science 2005 Jan;37(1):29-39
Journal of dermatological science 2005 Jan;37(1):29-39
Long-term maintenance of human keratinocytes in vitro.
Hasskarl J, Velupillai P, Piboonniyom SO, Grace M, Münger K
The Journal of investigative dermatology 2005 Feb;124(2):475-8
The Journal of investigative dermatology 2005 Feb;124(2):475-8
Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness.
Li DQ, Chen Z, Song XJ, de Paiva CS, Kim HS, Pflugfelder SC
Experimental eye research 2005 Apr;80(4):581-90
Experimental eye research 2005 Apr;80(4):581-90
Characterization of putative stem cell phenotype in human limbal epithelia.
Chen Z, de Paiva CS, Luo L, Kretzer FL, Pflugfelder SC, Li DQ
Stem cells (Dayton, Ohio) 2004;22(3):355-66
Stem cells (Dayton, Ohio) 2004;22(3):355-66
Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis.
Mizoguchi M, Suga Y, Sanmano B, Ikeda S, Ogawa H
Journal of dermatological science 2004 Sep;35(3):199-206
Journal of dermatological science 2004 Sep;35(3):199-206
Enhanced redundancy in Akt and mitogen-activated protein kinase-induced survival of malignant versus normal prostate epithelial cells.
Uzgare AR, Isaacs JT
Cancer research 2004 Sep 1;64(17):6190-9
Cancer research 2004 Sep 1;64(17):6190-9
Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency.
Hale LP, Buckley RH, Puck JM, Patel DD
Clinical immunology (Orlando, Fla.) 2004 Jan;110(1):63-70
Clinical immunology (Orlando, Fla.) 2004 Jan;110(1):63-70
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
Hale LP, Markert ML
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):617-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):617-24
Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.
D'Silva NJ, Mitra RS, Zhang Z, Kurnit DM, Babcock CR, Polverini PJ, Carey TE
Journal of cellular physiology 2003 Sep;196(3):532-40
Journal of cellular physiology 2003 Sep;196(3):532-40
Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels.
Hinterhuber G, Marquardt Y, Diem E, Rappersberger K, Wolff K, Foedinger D
Experimental dermatology 2002 Oct;11(5):413-20
Experimental dermatology 2002 Oct;11(5):413-20
Microtubule disruption in keratinocytes induces cell-cell adhesion through activation of endogenous E-cadherin.
Kee SH, Steinert PM
Molecular biology of the cell 2001 Jul;12(7):1983-93
Molecular biology of the cell 2001 Jul;12(7):1983-93
Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2.
Asai Y, Ohyama Y, Gen K, Ogawa T
Infection and immunity 2001 Dec;69(12):7387-95
Infection and immunity 2001 Dec;69(12):7387-95
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Involucrin using Involucrin Monoclonal Antibody (Product # MA5-11800) on LS174T Cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human skin stained with Involucrin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining in the epidermal region.
- Conjugate
- Biotin
