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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB600-717 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-717, RRID:AB_2139899
- Product name
- Rat Monoclonal MBP Antibody
- Antibody type
- Monoclonal
- Description
- Tissue culture supernatant. NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Guinea Pig, Porcine, Rabbit, Sheep
- Host
- Rat
- Antigen sequence
Amino acids 82-87.- Isotype
- IgG
- Vial size
- 1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Importance of Inter-Species Variation in Traumatic Brain Injury-Induced Alterations of Microglial-Axonal Interactions.
Sox2 Is Essential for Oligodendroglial Proliferation and Differentiation during Postnatal Brain Myelination and CNS Remyelination.
The Stem Cell Factor Sox2 Is a Positive Timer of Oligodendrocyte Development in the Postnatal Murine Spinal Cord.
Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig.
Quantitative proteomic analysis of the brainstem following lethal sarin exposure.
IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.
ZPK/DLK and MKK4 form the critical gateway to axotomy-induced motoneuron death in neonates.
A TSPO ligand is protective in a mouse model of multiple sclerosis.
Loss of osteoprotegerin expression in the inner ear causes degeneration of the cochlear nerve and sensorineural hearing loss.
Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.
IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory foci.
cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4).
Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after demyelination.
Low dose dextromethorphan attenuates moderate experimental autoimmune encephalomyelitis by inhibiting NOX2 and reducing peripheral immune cells infiltration in the spinal cord.
ZPK/DLK, a mitogen-activated protein kinase kinase kinase, is a critical mediator of programmed cell death of motoneurons.
Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.
Gorse KM, Lafrenaye AD
Frontiers in neurology 2018;9:778
Frontiers in neurology 2018;9:778
Sox2 Is Essential for Oligodendroglial Proliferation and Differentiation during Postnatal Brain Myelination and CNS Remyelination.
Zhang S, Zhu X, Gui X, Croteau C, Song L, Xu J, Wang A, Bannerman P, Guo F
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1802-1820
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1802-1820
The Stem Cell Factor Sox2 Is a Positive Timer of Oligodendrocyte Development in the Postnatal Murine Spinal Cord.
Zhang S, Rasai A, Wang Y, Xu J, Bannerman P, Erol D, Tsegaye D, Wang A, Soulika A, Zhan X, Guo F
Molecular neurobiology 2018 Dec;55(12):9001-9015
Molecular neurobiology 2018 Dec;55(12):9001-9015
Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pig.
Lafrenaye AD, Todani M, Walker SA, Povlishock JT
Journal of neuroinflammation 2015 Oct 6;12:186
Journal of neuroinflammation 2015 Oct 6;12:186
Quantitative proteomic analysis of the brainstem following lethal sarin exposure.
Meade ML, Hoffmann A, Makley MK, Snider TH, Schlager JJ, Gearhart JM
Brain research 2015 Jun 22;1611:101-13
Brain research 2015 Jun 22;1611:101-13
IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.
Pusic AD, Pusic KM, Clayton BL, Kraig RP
Journal of neuroimmunology 2014 Jan 15;266(1-2):12-23
Journal of neuroimmunology 2014 Jan 15;266(1-2):12-23
ZPK/DLK and MKK4 form the critical gateway to axotomy-induced motoneuron death in neonates.
Itoh T, Horiuchi M, Ikeda RH Jr, Xu J, Bannerman P, Pleasure D, Penninger JM, Tournier C, Itoh A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 6;34(32):10729-42
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 6;34(32):10729-42
A TSPO ligand is protective in a mouse model of multiple sclerosis.
Daugherty DJ, Selvaraj V, Chechneva OV, Liu XB, Pleasure DE, Deng W
EMBO molecular medicine 2013 Jun;5(6):891-903
EMBO molecular medicine 2013 Jun;5(6):891-903
Loss of osteoprotegerin expression in the inner ear causes degeneration of the cochlear nerve and sensorineural hearing loss.
Kao SY, Kempfle JS, Jensen JB, Perez-Fernandez D, Lysaght AC, Edge AS, Stankovic KM
Neurobiology of disease 2013 Aug;56:25-33
Neurobiology of disease 2013 Aug;56:25-33
Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.
Horiuchi M, Maezawa I, Itoh A, Wakayama K, Jin LW, Itoh T, Decarli C
Neurobiology of aging 2012 Mar;33(3):499-509
Neurobiology of aging 2012 Mar;33(3):499-509
IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory foci.
Lee E, Chanamara S, Pleasure D, Soulika AM
Journal of neuroinflammation 2012 Jan 16;9:7
Journal of neuroinflammation 2012 Jan 16;9:7
cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4).
Bertrand MJ, Lippens S, Staes A, Gilbert B, Roelandt R, De Medts J, Gevaert K, Declercq W, Vandenabeele P
PloS one 2011;6(9):e22356
PloS one 2011;6(9):e22356
Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after demyelination.
Soundarapandian MM, Selvaraj V, Lo UG, Golub MS, Feldman DH, Pleasure DE, Deng W
Scientific reports 2011;1:2
Scientific reports 2011;1:2
Low dose dextromethorphan attenuates moderate experimental autoimmune encephalomyelitis by inhibiting NOX2 and reducing peripheral immune cells infiltration in the spinal cord.
Chechneva OV, Mayrhofer F, Daugherty DJ, Pleasure DE, Hong JS, Deng W
Neurobiology of disease 2011 Oct;44(1):63-72
Neurobiology of disease 2011 Oct;44(1):63-72
ZPK/DLK, a mitogen-activated protein kinase kinase kinase, is a critical mediator of programmed cell death of motoneurons.
Itoh A, Horiuchi M, Wakayama K, Xu J, Bannerman P, Pleasure D, Itoh T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 May 18;31(20):7223-8
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 May 18;31(20):7223-8
Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.
Kawai K, Itoh T, Itoh A, Horiuchi M, Wakayama K, Bannerman P, Garbern JY, Pleasure D, Lindsten T
The European journal of neuroscience 2009 Dec 3;30(11):2030-41
The European journal of neuroscience 2009 Dec 3;30(11):2030-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
