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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-35330 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD4 in 293 cell lysate (2 µg/lane) either nontransfected (lane 1) or transiently transfected with the SMAD4 gene (lane 2) using a SMAD4 polyclonal antibody (Product # PA5-35330).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMAD4 showing staining in the cytoplasm and nucleus of Hela cells using a SMAD4 polyclonal antibody (Product # PA5-35330) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SMAD4 in formalin-fixed, paraffin-embedded human skeletal muscle tissue using a SMAD4 polyclonal antibody (Product # PA5-35330) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SMAD4 in MCF-7 cells (bottom) compared to a negative control (top) using a SMAD4 polyclonal antibody (Product # PA5-35330) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
