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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- ARG54741 - Provider product page
- Provider
- Arigo
- Product name
- anti-Smad4 antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein of human SMAD4 (NP_005350.1).
- Description
- Affinity purification with immunogen.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to weeks. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts of various cell lines stained with ARG54741 anti-Smad4 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells and DLD-1 cells stained with ARG54741 anti-Smad4 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U20S cell stained with ARG54741 anti-Smad4 antibody. Blue: DAPI for nuclear staining.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: SW480 cells stained with ARG54741 anti-Smad4 antibody (top, red) at 1:200 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cell stained with ARG54741 anti-Smad4 antibody. Blue: DAPI for nuclear staining.
