600-401-A41

antibody from Rockland Immunochemicals, Inc.

Targeting: SMAD4 DPC4, MADH4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-A41

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-A41, RRID:AB_2302323

Product name

Anti-SMAD4 (RABBIT) Antibody - 600-401-A41

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.

Validation comment

Western Blot