- 34-5600 - Invitrogen Antibodies BSG antibody

Submitted references CD147-targeted siRNA in A375 malignant melanoma cells induces the phosphorylation of EGFR and downregulates cdc25C and MEK phosphorylation.

Hatanaka M, Higashi Y, Kawai K, Su J, Zeng W, Chen X, Kanekura T
Oncology letters 2016 Apr;11(4):2424-2428

EMMPRIN co-expressed with matrix metalloproteinases predicts poor prognosis in patients with osteosarcoma.

Futamura N, Nishida Y, Urakawa H, Kozawa E, Ikuta K, Hamada S, Ishiguro N
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Jun;35(6):5159-65

Renal cell neoplasias: reversion-inducing cysteine-rich protein with Kazal motifs discriminates tumor subtypes, while extracellular matrix metalloproteinase inducer indicates prognosis.

Rabien A, Stephan C, Kilic E, Weichert W, Kristiansen G, Miller K, Jung K, Erbersdobler A
Journal of translational medicine 2013 Oct 16;11:258

Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.

Agrawal SM, Williamson J, Sharma R, Kebir H, Patel K, Prat A, Yong VW
Brain : a journal of neurology 2013 Jun;136(Pt 6):1760-77

High extracellular matrix metalloproteinase inducer/CD147 expression is strongly and independently associated with poor prognosis in colorectal cancer.

Stenzinger A, Wittschieber D, von Winterfeld M, Goeppert B, Kamphues C, Weichert W, Dietel M, Rabien A, Klauschen F
Human pathology 2012 Sep;43(9):1471-81

Decreased RECK and Increased EMMPRIN expression in urothelial carcinoma of the bladder are associated with tumor aggressiveness.

Wittschieber D, Stenzinger A, Klauschen F, Stephan C, Jung K, Erbersdobler A, Rabien A
Pathobiology : journal of immunopathology, molecular and cellular biology 2011;78(3):123-31

Multi-photon imaging of tumor cell invasion in an orthotopic mouse model of oral squamous cell carcinoma.

Gatesman Ammer A, Hayes KE, Martin KH, Zhang L, Spirou GA, Weed SA
Journal of visualized experiments : JoVE 2011 Jul 25;(53)

Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.

Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H874-86

EMMPRIN modulates migration and deposition of TN-C in oral squamous carcinoma.

Dang D, Atakilit A, Ramos DM
Anticancer research 2008 Jul-Aug;28(4B):2049-54

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BT-20 cells using Rb anti-EMMPRIN (Product # 34-5600).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts of SH-SY5Y (Lane 1), U-87 MG (Lane 2) and HeLa (Lane 3). The blots were probed with Anti- CD147/BSG Rabbit polyclonal Antibody (Product # 34-5600, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). In all cell lines tested, a band at 27 kDa was observed for the non-glycosylated form of CD147/BSG. Bands between 44kda-66kda were observed for the glycosylated form of CD147/BSG. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SRC was performed by loading 30 µg of HEK 293 (Lane 1), HEK 293- CD147 knockout (Lane 2) whole cell lysate. The blot was probed with Anti-CD147 Monoclonal Antibody (Product # 34-5600, 2 µg/mL) Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms antibody specificity to CD147.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD147/BSG was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD147/BSG Rabbit Polyclonal Antibody (Product # 34-5600) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD147 / BSG was done on NTERA-2 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with CD147 / BSG Rabbit Polyclonal Antibody (34-5600, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.